Monoclonal Antibodies
All antibodies are purified on a Protein A column (Ey et al., 1978)1 using the low-salt method.
The antibodies are supplied at a concentration requiring ~1:1000 dilution for Western blotting. All antibodies are stored in Phosphate Buffered Saline (PBS) pH 7.2. Store at -20°C. (stable for at least 12 months undiluted).
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7F11 Gyr A
This is an IgG2b monoclonal antibody raised against the N-terminal domain of E. coli Gyr A produced from tissue culture supernatant derived from mouse hybridoma cells.
Technical Documents
4D3 Gyr A
This is an IgG2b monoclonal antibody raised against the C-terminal domain of E. coli Gyr A produced from tissue culture supernatant derived from mouse hybridoma cells.
Technical Documents
9G8 Gyr B
This is an IgG2a monoclonal antibody raised against the C-terminal domain of E. coli Gyr B produced from tissue culture supernatant derived from mouse hybridoma cells.
Technical Documents
7D3 Gyr B
This is an IgG2a monoclonal antibody raised against the N-terminal domain of E. coli Gyr B produced from tissue culture supernatant derived from mouse hybridoma cells.
Technical Documents
References
- Ey, P.L., S.J., & Jenkin, C.R. (1978). Isolation of pure IgG1, IgG2a, and IgG2b immunoglobulins from mouse serum using Protein A sepharose. Immunochemistry. 15: 429-436
- Harlow, E. & Lane, D (1988). Antibodies: a laboratory manual. Cold Spring Harbor Laboratory press. Cold Spring Harbor
