Trabecular meshwork is present around the circumference of the root of the iris and has a slit opening into the Schlemm’s canal that drains aqueous to venous vessels. Human Trabecular meshwork cells (TMC) play an active role in the aqueous humor outflow and this pathway is intraocular pressure-dependent. Injury or death of TMC, therefore, is related with the pathogenesis of open-angle glaucoma. TMC further express receptors for neurotransmitters and neuropeptides. THey respond to very low concentrations of a variety of vasoactive peptides and growth factors, allowing these cells to regulate the permeability of trabecular meshwork at multiple levels. TMC cultures provides an invaluable tool to study the functional control of trabecular meshwork. Human Trabecular Meshwork Cells will encourage the development of more efficient strategies to treat glaucoma. They are also very useful cells to stablish disease models for High Throughput and Hight Content Screening.