| ELISA 2.0 kit for measuring Bos domesticus β-casein allergen, Bos d 11. | ||||
| Antibodies: | CC11/VBIC | |||
| Standard Range: | 1,000-1.95ng/mL | |||
| Limit of Detection: | 7.81ng/mL | |||
| Background: | OD<0.08 at 450nm | |||
| Coefficient of Determination: | R-squared>0.98 | |||
| Contents: | Plate: Pre-coated with anti-Bos d 11 monoclonal antibody CC11Vial 1: (white top) Bos d 11 allergen standardConcentration: 10,000ng/mlVial 2: (brown) Biotinylated monoclonal antibody VBICVial 3: (blue top) Streptavidin-peroxidaseBottle 1: Wash buffer, (10x concentrate)Bottle 2: Assay buffer, (10x concentrate)Bottle 3: TMB developing substrateBottle 4: Stop solution (0.5N sulfuric acid) | |||
| Storage: | Store at 2-8°C | |||
| Expiry: | 6 months from date of receipt | |||
| Not Provided: | Type I ultrapure water or 18.2MΩ de-ionized waterVolumetric measuring equipment (e.g. serological pipette, graduated cylinder)Clean containers for buffer and reagent preparationCalibrated single and multi-channel micropipettes and tipsVortex mixerPlate reader capable of reading absorbance at 450nmAnalysis software (recommended, but not required) | |||
| Notes: | The allergen standard is recommended for immunoassay calibrationpurposes only.A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website. | |||
| Product Resources: | EPC-BD11-1 Certificate of Analysis Validation Performance Data | |||
For research and commercial use in vitro:not for human in vivo or therapeutic use. | ||||
| Certificate of Analysis | ||||
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated withmonoclonal antibody CC11 and treated with stabilizingagent. Sealed in foil pouch with desiccant. | |||
| Monoclonal Antibody: | CC11 | |||
| Immunogen: | Bovine β-casein allergen | |||
| Isotype: | IgG1 | |||
| Specificity: | Binds to an epitopeonbovine Bos domesticus β-casein allergen, Bos d 11. | |||
| Purification: | Produced in ascites and purified by affinity chromatography. Single heavy and light chain bands on SDS-PAGE. | |||
| Certificate of Analysis | ||||
| Detection Antibody: | VBIC | |||
| Immunogen: | Bovineβ-casein allergen | |||
| Isotype: | IgG1 | |||
| Specificity: | Binds to an epitope on bovine Bos domesticus β-casein allergen, Bos d 11. | |||
| Purification: | Produced in ascites and purified by affinity chromatography. Single heavy and light chain bands on SDS-PAGE. | |||
| Biotinylation: | Biotinylated and titrated for use in ELISA 2.0 at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free. | |||
| Certificate of Analysis | ||||
| Allergen Standard: | Natural Bos d 11 allergen was prepared in 1% BSA/ 50% glycerol/PBS, pH 7.4. . | |||
| Concentration: | 10,000ng/mL (based on amino acid analysis) | |||
References: 1. Luyt D, Ball H, Makwana N, Green MR, Bravin K, et al. BSACI Guideline for the Diagnosis and Management of Cow"s Milk Allergy. Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology. 2014. 2. Fiocchi A, Bouygue GR, Albarini M, Restani P. Molecular diagnosis of cow’s milk allergy. Curr Opin Allergy Clin Immunol. 2011 Jun;11(3):216-21. | ||||
