ELISA 2.0 kit for measuring Arachis hypogaea allergen, Ara h 1. | |||||||
| Antibodies: | 2C12/2F7 | ||||||
| Standard Range: | 2,000-4.0 ng/mL | ||||||
| Limit of Detection: | 31.5 ng/mL | ||||||
| Background: | OD<0.08 at 450nm | ||||||
| Coefficient of Determination: | R-squared >0.98 | ||||||
| Contents: | Plate: Pre-coated with anti-Ara h 1 monoclonal antibody 2C12Vial 1: (white top) Ara h 1 allergen standardConcentration: 20,000 ng/mlVial 2: (brown) anti-Ara h 1 antibody 2F7Vial 3: (blue top) Streptavidin-PeroxidaseBottle 1: Wash buffer, (10x concentrate)Bottle 2: Assay buffer, (10x concentrate)Bottle 3: TMB developing substrateBottle 4: Stop solution (0.5N sulfuric acid) | ||||||
| Storage: | The ELISA 2.0 kit should be stored at 2-8°C. | ||||||
| Expiry: | 6 months from date of receipt | ||||||
| Not Provided: | Type I ultrapure water or 18.2MΩ de-ionized waterVolumetric measuring equipment (e.g. serological pipette, graduated cylinder)Clean containers for buffer and reagent preparationCalibrated single and multi-channel micropipettes and tipsVortex mixerPlate reader capable of reading absorbance at 450nmAnalysis software (recommended, but not required) | ||||||
| Notes: | The allergen standard is recommended for immunoassay calibrationpurposes only.A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website. | ||||||
| Product Resources: | EPC-AH1-1 Certificate of Analysis Validation Performance Data | ||||||
For research and commercial use in vitro:not for human in vivo or therapeutic use. | |||||||
| Certificate of Analysis | |||||||
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated withmonoclonal antibody 2C12 and treated with stabilizingagent. Sealed in foil pouch with desiccant. | ||||||
| Monoclonal Antibody: | 2C12 | ||||||
| Immunogen: | Ara h 1 | ||||||
| Isotype: | Mouse IgG1 | ||||||
| Specificity: | Binds to species specific epitope present on Arachis hypogaea allergen, Ara h 1. | ||||||
| Purification: | Produced in vitro by BioVectra dcl bioreactors and purified by chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE. | ||||||
| Certificate of Analysis | |||||||
| Detection Antibody: | 2F7 | ||||||
| Immunogen: | Ara h 1 | ||||||
| Isotype | Mouse IgG1 | ||||||
| Specificity: | Binds to species specific epitope present onArachis hypogaeaallergen, Ara h 1. | ||||||
| Activity: | Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free. | ||||||
| Certificate of Analysis | |||||||
| Allergen Standard: | Purified natural Ara h 1 prepared in 1% BSA/50%glycerol/PBS, pH 7.4. | ||||||
| Concentration/Calibration: | 20,000 ng/mL (based on amino acid analysis) | ||||||
| References: | |||||||
| 1. Pomés A, Helm RM, Bannon GA, Burks AW, Tsay A, Chapman MD. Monitoring peanut allergen in food products by measuring Ara h 1. J Allergy Clin Immunol 2003;111:640-5. | |||||||
2. Pomés A, Vinton R, Chapman MD. Peanut allergen (Ara h 1) detection in foods containing chocolate. J Food Prot. 2004 Apr:67 (4):793-8. 3. Perry TT, Conover-Walker MK, Pomés A, Chapman MD, Wood RA. Distribution of peanut allergen in the environment. J Allergy Clin Immunol. 2004 May;113(5):973-6. 4. Maloney JM, Chapman MD, Sicherer SH. Peanut allergen exposure through saliva: assessment and interventions to reduce exposure. J Allergy Clin Immunol. 2006 Sep;118(3):719-24. | |||||||
