| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name | Fast Histamine ELISA kit - Fish samples - HistaSure™ |
| Description | Fast-track Enzyme immunoassay (ELISA) for the quantitative determination of histamine levels in fresh fish, frozen fish, canned fish or fish meals. |
| Labels | AOAC Performance Tested Method #021402 |
| Format | 48-well plate |
| Samples | 10g fish sample |
| Cross specie-reactivity | React with all species |
| Standard range | 0 / 3.0 - 300 ppm |
| Sensitivity | 0.44 ppm |
| Specificity | No significant cross-reactivity was observed with Histamine analogs such as L-Tryptophan, Tryptamine, 3-Methyl-Histamine, L-Histidine, L-Tyrosine, L-Phenylalanine, Tyramine, Cadaverine, Spermine, Putrescine, Trimethylamine. |
| Assay time | Sample acylation 5 min, ELISA 20 min |
| Storage | Store at 2-8°C for up to 12 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample collection & storage | Fresh/Frozen fish: keep fish frozen until analysis. Thaw samples under refrigeration or in cold water (no heated water bath). Store samples refrigerated (2-8°C) prior to testing. Blend samples until homogeneous. Canned fish: Blend can content until homogeneous. Canned marine products packed in oil, sauce, brine or broth: Drain for 2 minutes and blend until homogeneous. Fish meal: Blend sample until homogeneous. |
| Extraction | Weigh 10 g of sample, add 240 ml ultra-pure water and homogenize for 1-2 minutes with blender. Filter/centrifuge homogeneate. Use 50µl of extract for acylation. |
| Acylation | Pipette 50µl of control(s) and sample extracts in wells. Add acylation buffer. Incubate with acylation reagent for 5min at RT on a shaker. |
| ELISA | Histamine antiserum incubation (10 min), revelation and read steps (20 min). |
| Detailed protocol | Download instructions for use |
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