Product information | Key steps | References | Technical support | Reviews |
Product overview
Product name | 5-HIAA ELISA kit - Urine - Fast-Track |
Description | In vitro diagnostics enzyme immunoassay (ELISA) for the fast determination of 5-HIAA in urine samples. |
Labels | IVD, CE |
Format | 96-well plate |
Samples | 50 µl urine |
Cross specie-reactivity | React with all species |
Standard range | 0 / 0.5 - 50 mg/l |
Sensitivity | 0.17 mg/l |
Specificity | No significant cross-reactivity was observed with 5-HIAA analogs such as Serotonin, 5-Hydroxy-DL-Tryptophan, Tryptamine, Melatonin, 5-Hydroxytryptamin, Vanillic mandelic acid and Homovanillic Acid. |
Assay time | 3 hours |
Storage | Store at 2-8°C for up to 6 months. |
Datasheets | Instructions for use , Material safety datasheet |
Key steps
Sample collection & storage | Spontaneous or 24-hour urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C. |
Sample preparation | Sample predilution and methylation (30 min) |
ELISA | 5-HIAA antiserum incubation (1 hour), revelation and read steps (90 min). |
Detailed protocol | Download instructions for use |
References
- Beer TM et al. Acupuncture for Hot Flashes in Patients With Prostate Cancer. Urology. 2010 Nov;76(5):1182-8.
- Jackson LN et al. Development and Characterization of a Novel In vivo Model of Carcinoid Syndrome. Clin Cancer Res. 2009 Apr 15;15(8):2747-55.
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