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商品描述
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name | Tryptophan ELISA kit |
| Description | In vitro diagnostic enzyme immunoassay (ELISA) for the quantitative determination of L-Tryptophan in biological fluids (urine, plasma, serum samples, ...) and cell culture supernatants. |
| Labels | IVD, CE |
| Format | 96-well plate |
| Samples | urine, plasma, serum, cell culture supernatant |
| Sample volume | 20 µL |
| Cross species-reactivity | React with all species |
| Standard range | 2.5 - 250 µg/ml |
| Sensitivity | < 1.2µg/ml |
| Specificity | No significant cross-reactivity was observed with L-Tryptophan analogs such as 5-Hydroxy-L-tryptophan, Tryptamine, 5-Methoxytryptophan, 5-Hydroxytryptamine, N-acetyl-5-hydroxytryptamine. |
| Assay time | Sample preparation 2.5 hours and ELISA overnight |
| Storage | Store at 2-8°C for to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample collection & storage | Urine: spontaneous or 24-hour urine collected in bottle with 10-15 mL of 6 M HCl, stored at -20°C for long periods (up to 6 months)Plasma/Serum: EDTA-, Heparin- or Citrate- samples stored at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). Do not use haemolytic or lipemic samples. |
| Sample preparation | Sample precipitation (15 min) and derivatization (2h15) |
| ELISA | Tryptophan antiserum overnight incubation, revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
PrProduct citations :
- Watanabe et al.Addding indoximod to hypofractionated radiotherapy with anti-PD-1 checkpoint blockade enhances early NK and CD8+ T cell-dependent tumor activity. Clin Cancer Res. 2019 Nov 6
- Walker et al. Leucine competes with kynurenine for blood-to-brain transport and prevents lipopolysaccharide-induced depression-like behavior in mice. Mol Psychiatry. 2018 Jul 9.
- Ziklo et al. Dysbiosis of the Vaginal Microbiota and Higher Vaginal Kynurenine/Tryptophan Ratio Reveals an Association with Chlamydia trachomatis Genital Infections. Front Cell Infect Microbiol. 2018 Jan 18.
- Gautam et al. In vivo inhibition of tryptophan catabolism reorganizes the tuberculoma and augments immune-mediated control of Mycobacterium tuberculosis. Proc Natl Acad Sci U S A. 2018 Jan 2
- Martiin-Hernandez et al. Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats. Mol Neurobiol. 2018 May 3
- Toulmonde et al. Use of PD-1 Targeting, Macrophage Infiltration, and IDO Pathway Activation in Sarcomas. JAMA Oncol. 2018 Jan 1;4
- Ziklo et al. In vitro rescue of genital strains of Chlamydia trachomatis from interferon-γ and tryptophan depletion with indole-positive, but not indole-negative Prevotella spp. BMC Microbiol. 2016 Dec 3.
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