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商品描述
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name | Histamine ELISA kit - Urine and Plasma samples |
| Description | In vitro diagnostics enzyme immunoassay (ELISA) for the quantitative determination of histamine levels in urine and plasma samples. |
| Labels | IVD, CE |
| Format | 96-well plate |
| Samples | 10 µl urine, 25 µl plasma |
| Cross specie-reactivity | React with all species |
| Standard range | 0 / 0.5 - 50 ng/ml |
| Sensitivity | 0.12 ng/ml plasma, 0.3 ng/ml urine |
| Specificity | No significant cross-reactivity was observed with Histamine analogs such as 3-Methyl-Histamine, Tyramine, L-Phenylalanine, L-Histidine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin |
| Assay time | 1h sample acetylation + ELISA overnight |
| Storage | Store at 2-8°C for up to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample collection & storage | Plasma: EDTA or Heparin plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 - 8°C; for longer periods (up to 6 months) at - 20°C. Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C. |
| Sample preparation | Sample acylation (1h) |
| ELISA | Histamine antiserum incubation (3H or overnight), revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
- Product citation: Blanchini et al. Inactivated antithombin as anticoagulant reversal in a rat model of cardiopulmonary bypass: a potent and potentially safer alternative to protamine. Br J Haematol. 2018 Mar;180(5).
- Yagcı M et al. TCTP/HRF pathway and angiogenesis: a feasible intercourse in chronic lymphocytic leukemia. Leuk Res. 2013 Jun;37(6):665-70.
- Boskabady MH, Tabatabaee A, Byrami G. The effect of the extract of Crocus sativus and its constituent safranal, on lung pathology and lung inflammation of ovalbumin sensitized guinea-pigs. Phytomedicine. 2012 Jul 15;19(10):904-11.
- Martin SK et al. Influence of Dietary Histidine on Basophil Release, Circulating Concentration, and Urinary Excretion of Histamine in Domestic Felines. Intern J Appl Res Vet Med. 2012. 10: 289-299
- McCord JL et al. H2-receptor-mediated vasodilation contributes to postexercise hypotension. J Appl Physiol (1985). 2006 Jan;100(1):67-75.
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