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商品描述
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name | Histamine ELISA kit - Any sample - Research |
| Description | Enzyme immunoassay (ELISA) for the quantitative determination of histamine levels in biological samples. Research use only. |
| Format | 96-well plate |
| Samples | 10 µl, any sample |
| Cross specie-reactivity | React with all species |
| Standard range | 0 / 0.5 - 50 ng/ml |
| Sensitivity | 0.2 ng/ml |
| Specificity | No significant cross-reactivity was observed with Histamine analogs such as 3-Methyl-Histamine, Tyramine, L-Phenylalanine, L-Histidine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin |
| Assay time | 1h30 sample acetylation + ELISA overnight |
| Storage | Store at 2-8°C for up to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample preparation | Sample predilution and acylation (1h30) |
| ELISA | Histamine antiserum incubation (3H or overnight), revelation and read steps (1h30). |
| Detailed protocol | Download instructions for use |
References
Product citation:
- Product citation: von Beek et al. Streptococcal sagA activates a proinflammatory response in mast cells by a sublytic mechanism. Cell Microbiol. 2019 Sep
- Product citation: Heidinger et al. Decreased viability and proliferation of Chang conjunctival epithelial cells after contact with ultraviolet light-irradiated pollen. Cutaneous and Ocular Toxicology. 2018 Sep;37(3):210-217.
- Bertl K et al. Smoking influences salivary histamine levels in periodontal disease. Oral Dis. 2012 May;18(4):410-6.
- Han SC. et al. Fermented fish oil suppresses T helper 1/2 cell response in a mouse model of atopic dermatitis via generation of CD4+CD25+Foxp3+ T cells. BMC Immunology 2012, 13:44
- Yang M, Yang C, Mine Y. Multiple T cell epitope peptides suppress allergic responses in an egg allergy mouse model by the elicitation of forkhead box transcription factor 3- and transforming growth factor-beta-associated mechanisms. Clin Exp Allergy. 2010 Apr;40(4):668-78.
- Husain M, Boermans HJ, Karrow NA. Mesenteric lymph node transcriptome profiles in BALB/c mice sensitized to three common food. BMC Genomics. 2011 Jan 6;12:12.
- Yang M et al. Immunomodulatory effects of egg white enzymatic hydrolysates containing immunodominant epitopes in a BALB/c mouse model of egg allergy. J Agric Food Chem. 2009 Mar 25;57(6):2241-8.
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