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商品描述
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name | Epinephrine ELISA kit - Research |
| Description | Enzyme immunoassay (ELISA) for the ultra-sensitive determination of epinephrine in biological samples. Research use only. |
| Format | 96-well plate |
| Samples | Any sample |
| Sample volume | 1 - 750 µl |
| Cross specie-reactivity | React with all species |
| Standard range | 0 / 0.5 - 80 ng/ml |
| Sensitivity | 3.3 pg/ml |
| Specificity | No significant cross-reactivity was observed with Adrenaline analogs such as Dopamine, Noradrenaline, Metanephrine, Normetanephrine, Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid. |
| Assay time | Sample preparation 4 hours and ELISA overnight |
| Storage | Store at 2-8°C for to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample preparation | Sample extraction and acylation (1h45), enzymatic conversion (2h) |
| ELISA | Epinephrine antiserum overnight incubation, revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
- Eickmeier O et al. Aspirin-triggered resolvin D1 reduces mucosal inflammation and promotes resolution in a murine model of acute lung injury. Mucosal Immunol. 2013 Mar;6(2):256-66.
- Knight CM et al. Mediobasal hypothalamic SIRT1 is essential for resveratrol"s effects on insulin action in rats. Diabetes. 2011 Nov;60(11):2691-700.
- Pullar CE et al. beta-Adrenergic receptor antagonists accelerate skin wound healing: evidence for a catecholamine synthesis network in the epidermis. J Biol Chem. 2006 Jul 28;281(30):21225-35.
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