Product Specifications:

Item # 1001-F:  Fluorescene rgp120 HIV-1 IIIB

 

Mass/vial: Approximately 50µg gp120                                  

 

Diluent: 10mM Tris, pH 8.0                                  

 

Stabilizer: None                                                       

 

Preservative: None                                                       

 

Storage: -75°C                                                      

 

Physical State: Frozen Liquid                                           

 

F/P Ratio: 4 -5

 

Stability: 6 months at -75°C

 

Minimum Dilution: 1 : 50

 

Applications: Direct Immunofluorescence, Immunohistocytology, FACS, Dot Blots

 

Description: Baculovirus derived rgp120 HIV-1 IIIB conjugated to FITC.

 

Purification: Fluorescene conjugated rgp120 was purified by gel exclusion chromatography and UF concentrated.

 

Specificity: This fluorescene conjugated gp120 binds to murine monoclonal antibodies of defined epitope specificity.

 

Biological Activity: This fluorescene conjugated gp120 binds to CD4 bearing T-cells  as determined by  FACS.

 

Application and Instruction for use:

 

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining may be performed in 1 : 50 dilution range.  Dot Blot assays with purified anti-gp120 monoclonal/ rabbit polyclonal antibodies may be performed at a minimum dilution of 1 : 100.

 

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