Product Specifications:

Item# 1131-B: Biotin Murine Anti-gp120 HIV-1 YU2 mAb IgG

 

Mass/vial: Approximately 50µg IgG 

 

Volume: 50µl  

 

Diluent: PBS

 

Stabilizer: 2.5% BSA

 

Preservative: 0.02% Azide

 

Storage: 4°C

 

Physical State: Liquid

 

B/P Ratio: 10 - 20

 

Stability: At least 6 months at 4°C

 

Minimum Dilution: 1 : 5000

 

Applications: ELISA, Westerns, FACS, Immunohistopcytology.

 

Description: Biotin conjugated Murine anti-gp120 (HIV-1) YU2 M Tropic mAb

 

Purification: Highly purified mAb IgG (>95% pure) was used for Biotin conjugation and the conjugate purified by gel exclusion chromatography.

 

Specificity: This biotin conjugated mAb binds to native and recombinant HIV-1 gp120 YU2 M Tropic and HIV-1 MN ( Dual Tropic ), but not to HIV-1 ADA M Tropic, in Dot Blot assays, ELISA and Western ELISA.

 

Biological Activity: None

 

Application and Instructions for use: 

Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed in 1 : 5000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. Western ELISA may be performed in 10-100pg gp120 range with 0.1-0.5µg/ml conjugate.

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