Product Specifications: 

Item # 2503: Human Anti-p24 HIV-1 IIIB pAb IgG

 Concentration: See vial 

 Mass/vial: 100ug

 Diluent: PBS 

 Purity: >90% 

 Stabilizer: None

 Preservative: None 

 Storage: -75°C 

 Physical State: Frozen Liquid

 Stability: At least 6 months at -75°C

 Minimum Dilution: 1 : 1000

 Application: ELISA/p24 Western ELISA, Immunoprecipitation,  Immunohistochemistry, Virus Neutralization Assays, FACS, p24 Capture ELISA.

 Description: pAb IgG fraction purified from HIV-1 immune human serum .

 Purification: This pAb IgG was purified by rp24 affinity chromatography to >95% purity as determined by SDS-PAGE, reduced.

 Specificity: This pAb IgG binds to native and recombinant HIV-1 p24 in ELISA and Western ELISA.

 Biological Activity: None

 Application and Instructions for use:

Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. This pAb IgG yields titres of greater than 1 : 2000 with 100ng of immobilized p24 (IDI Product# 1003) under standard conditions. ELISA and Western ELISA may be performed at 1-2µg/ml of pAb depending on the nature and the quantity of the immobilized antigen. Histochemical studies require dilutions in 1 : 200 dilution range.

 Caution: Avoid the use of needles and ensure proper laboratory safety precautions are in place for the use of this product.

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