Product Specifictions

Item#1089: Recombinant HIV-1 ADA ( M-Tropic) Envelope Protein gp41 (E.coli).

 Concentration: See vial 

 Mass/vial: 0.1mg

 Volume/vial: See Vial

 Diluent: 10 mM Tris, pH 8.0, SDS (<0.01%)

 Purity : >99%

 Stabilizer: None

 Preservative: None

 Storage: -75°C

 Physical State: Frozen Liquid

 Stability: At least 24 months at -75oC.

 Applications: In-Vitro Diagnostics, Immunization, T-Cell Activation

 Description: Truncated Recombinant HIV-1 ADA M-Tropic envelope protein gp41 produced as a fusion protein in the E.coli Expression System. Truncated gp41 protein mol.wt: 25kD.

 Purification: This protein is purified by affinity chromatography and by preparative electrophoresis to >99% purity as determined by SDS-PAGE, reduced.

 Specificity: This protein binds to murine anti-gp41 mAbs and HIV-1 converted human serum polyclonal antibodies in ELISA and Western Elisa.

 Biological activity: T-cell activation assays pending.

 Application and instructions for use

Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 0.1 – 0.5mg/ml.

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