The antibody has a proven strong Biotin-Streptavidin/HRP staining at a 1/500-1/1000 dilution, in rat adrenal medulla and rat stomach.
Photo Description: IHC image of the rat adrenal medulla staining for SP-1 chomogranin A (porcine) with FITC-conjugated secondary antibody (above) and with standard biotin-streptavidin/HRP and DAB chromogen (below). The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:1000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS.
Host:Rabbit
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Mouse, RatAvailability: In Stock
Alternate Names: Pituitary secretory protein I; CgA; chromogranin A (parathyroid secretory protein 1), anti-SP-1 Chromogranin A
Gene Symbol: CHGARRID: AB_572226Database Links:Entrez Gene:102413232 BuffaloEntrez Gene:423420 ChickenEntrez Gene: 281070CowEntrez Gene:101318300 DolphinEntrez Gene:1113HumanEntrez Gene:397540MouseEntrez Gene:403993 PigEntrez Gene:101114907 Ram (Sheep)Entrez Gene:24258 Rat
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