The antibody has a proven strong biotin-streptavidin/HRP staining at a 1/1000 - 1/2000 dilution in rat globus pallidus and spinal cord. Staining is completely eliminated by pretreatment with 50 µg of Leucine Enkephalin per mL of diluted antiserum. Pretreatment with 50 µg of Methionine Enkephalin per mL of diluted antiserum also significantly blocks staining.
Photo Description: Low magnification IHC image of leucine enkephalin staining in the rat globus pallidus. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Host:Rabbit
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Bird, Bovine, Bullfrog, Cat, Chick, Dog, Fish, Frog, Guinea Pig, Hamster, Human, Lungfish, Monkey, Mouse, Octopus, Pig, Pigeon, Rat, Ray, Shark, Sheep, Starling, Three-Spined Stickleback, Turtle, Zebra Finch
Availability: In Stock
Alternate Names: Enkephalin (Leu+Met); Proenkephalin-A; enkephalin A; preproenkephalin, anti-Leucine Enkephaline, Leu Enk
Gene Symbol: PENKRRID: AB_572247Database Links:Entrez Gene:281387BovineEntrez Gene:768277CatEntrez Gene:421131ChickEntrez Gene:100687307 DogEntrez Gene:548461 FrogEntrez Gene:100379621 Guinea PigEntrez Gene:101844627HamsterEntrez Gene: 5179HumanEntrez Gene:696649MonkeyEntrez Gene:18619 MouseEntrez Gene:100152093 PigEntrez Gene:102088743 PigeonEntrez Gene:29237 RatEntrez Gene:103177054 SharkEntrez Gene:101109024 SheepEntrez Gene:106851221 StarlingEntrez Gene: 102933698 TurtleEntrez Gene:100190068 Zebra Finch
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