The antibody has a proven strong biotin-streptavidin/HRP staining at a 1/2000 - 1/4000 dilution in rat brainstem, cerebellum and adrenal medulla.  The above image is of DBH positive neurons in rat brainstem,  vibratome sectioned, ABC / DAB detection.Using Western blot of purified DBH the antiserum detects a triplet at approximately 72-74 kD. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.

Photo Description: IHC image of neurons staining for DBH in the rat brainstem. The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:4000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Species Reactivity: Bird, Cat, Ferret, Finch, Guinea Pig, Hamster, Hatchetfish, Human, Ilyanassa Obsoleta (Sea Snail), Monkey, Mouse, Pig, Quail, Rat, Sparrow, Starling, Steer (Cattle), Turkey, Turtle, Zebra Finch

Availability: In Stock

Alternate Names:  DBM; DOPO; Dopamine beta-monooxygenase, anti-DBH

RRID:  AB_572229Immunogen: Bovine DBHGene Symbol:  DBHDatabase Links:  Entrez Gene:  101082957   Cat  Entrez Gene:  101687995   Ferrett  Entrez Gene:  102038113   Finch  Entrez Gene:  100727873   Guuinea Pag  Entrez Gene:  100761411   Hamster  Entrez Gene:  1621            Human    Entrez Gene:  574105        Monkey  Entrez Gene:  13166          Mouse  Entrez Gene:  733609        Pig  Entrez Gene:  107321868  Quail  Entrez Gene: 25699           Rat  Entrez Gene: 102067155   Sparrow  Entrez Gene: 106851290   Starling  Entrez Gene:  280758       Steer (Cattle)  Entrez Gene: 100544779   Turkey  Entrez Gene: 102455557   Turtle  Entrez Gene: 100224356   Zebra Finch (Bird)

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