The antibody has a provenmaximum biotin-streptavidin/HRP staining at a 1/1000 - 1/2000 dilution in rat raphe nuclei. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Shipper and Tilders, 1983). Results showed that the 5-HTP antibody dose dependently stained 5-HTP but did not stain any concentration of 5-HT or 5-HIAA. The antiserum was also tested by pre-adsorption with indole/paraformaldehyde/BSA conjugates. Staining was completely blocked by pre-adsorption with 5-HTP conjugate and unaffected by 5-HIAA or 5-HT conjugate.Photo Description: IHC image of neurons staining for 5-HTP in the raphe nucleus of the rat brainstem. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Host: Rabbit

Quantity / Volume: 100 µL

State: Lyophilized Whole Serum

Reacts With: Rat, Sea Slug (Aplysia Californica, Tritonia Diomedea

Availability: In StockAlternate Names: anti-5-HTPRRID: AB_572215Immunogen: 5-HTPGene Symbol: AvpEntrez Gene ID: 24221NCBI Gene Aliases: DI, Va

Immuno Star我们的抗体研究客户包括美国和世界各地的主要大学，医院，公司，政府和其他机构神经科学家。我们的免疫组化产品可用于检测多种生物标记。这些疾病包括帕金森氏病，阿尔茨海默氏病，癫痫和脑癫痫，精神分裂症，楼格里格氏病和亨廷顿氏病。我们的产品用于鸦片受体的研究，具有改善从酒精中毒到类风湿关节炎的治疗方法的潜力。我们的科学团队致力于质量控制测试。这样可以确保我们所有的抗体在批次间均具有可靠性。我们对质量的执着使我们的产品在神经科学研究人员中享有极高的声誉，这些研究人员知道并相信ImmunoStar的抗体有效。