GB-Mount is a water-based mounting media for using with frozen section, immunoperoxidase and immunofluorescent procedures. AEC, AP-Red, and AP-Blue are soluble in alcohol and organic solvents. Tissue slides processed by these methods should be coverslipped with GB-Mount. Immunofluorescent assays require a mounting media that will not interfere with the assay results. When appropriate filters used, GB-Mount will not show any fluorescence background. Besides, there is no risk of quenching fluorescent emission. GB-Mount is supplied in a 18 ml a dropper bottle or 100ml bottle.
Recommended Protocol: 1. After saline rinse, shake off excess liquid from the slide and quickly wipe the back of the slide. 2. Invert dropper bottle to displace bubbles from the tip-end. 3. Squeeze out the first drop onto a paper towel to remove of air bubble on the tip. 4. Add a few drops of GB-Mount on the tissue section on the slide. 5. Carefully lay down coverglass to cover the tissue section. 6. Gently press out any noticeable air bubbles. If there are many bubbles on the slide, one may soak the slide in saline, remove the coverslip, and repeat the coverslipping procedure 7. The slide may be viewed immediately after coverslipping, be careful not to dislodge the coverslip before the slide has dried. 8. Allow sufficient time of drying slides before storage. 9. For maximum retention of fluorescence, slides should be kept in dark place at 4°C.
IHCWORLD的免疫组织化学染色的一种更加用户友好的方法。该托盘不仅适合需要潮湿箱的常规染色,而且是血液学,细胞学和微生物学实验室的理想选择。只用一只手就能使操作安全,容易。该StainTray™具有由坚韧的ABS塑料承受宽范围的化学品的黑色基。 当需要湿度时,轨道之间的井将牢固地容纳水而不会飞溅。塑料托盘上覆盖有聚合物条,即使将托盘倾斜放置,也能 完美固定幻灯片。提起滑轨不仅可以避免水接触滑片,还可以使滑片更容易取回。底座还将容纳从载玻片上滴落的多余污渍溶液。可以卸下排放塞,以更轻松地排空StainTray 。 四个橡胶支脚确保了更大的基础稳定性。单元可堆叠以节省空间。 提供两种封面:一个透明的盖子,便于目测。由PETG制成,温度范围为 -20ºC至60ºC。 一个黑色盖子荧光灯的工作。由ABS制成,温度范围为-80ºC至80ºC。