Description | Annexin I, also knows as lipocortin I (Lipo1), belongs to the family of annexins. These proteins are thought to control the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes. In two lipocortins (I and II) a short amino-terminal sequence distinct from the core structure has potential regulatory functions which are dependent on its phosphorylation state. The gene in the mouse encodes a protein of 346 amino acid residues. Mouse Lipo1 gene spans about 17 kb and is divided into 13 exons. Annexin I gene, mapped to 9q11-q22, is located on mouse chromosome 19. Annexin I acts through the formyl peptide receptor on human neutrophils. Peptides derived from the unique N-terminal domain of annexin I serve as FPR ligands and trigger different signaling pathways in a dose-dependent manner. |
Catalog Number | IW-PA1006 |
Quantity | 9 ml |
Host | Rabbit |
Clone | Polyclonal |
Isotype | IgG |
Immunogen | A synthetic peptide corresponding to a sequence near the C-terminal of human Annexin I, different from the related rat and mouse sequence by two amino acids. |
Purity | Immunogen affinity purified |
Conjugate | Unconjugated |
Species Reactivity | Human, mouse, rat. Not tested in other species. |
Positive Control | Liver, skin, cardiac muscle. |
Cellular Localization | Membrane, cytoplasmic or nuclear |
Form | Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted. |
Storage | Store at 2-8 °C. Do not freeze. |
Applications | IHC-P: Heat induced epitope retrieval (HIER) is required on formalin fixed paraffin sections. IHC-Tek Epitope Retrieval Solution (IW-1100) is recommended. IHC-Fr: Not tested. ICC: Suitable |
Limitations | This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user. |
Precautions | Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes. |
References | 1. Wallner, B. P.; Mattaliano, R. J.; Hession, C.; Cate, R. L.; Tizard, R.; Sinclair, L. K.; Foeller, C.; Chow, E. P.; Browning, J. L.; Ramachandran, K. L.; Pepinsky, R. B. : Cloning and expression of human lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activity. Nature 320: 77-81, 1986. . 2. Kovacic RT, Tizard R, Cate RL, Frey AZ, Wallner BP. Correlation of gene and protein structure of rat and human lipocortin I. Biochemistry. 1991 Sep 17;30(37):9015-21. 3. Walther, A.; Riehemann, K.; Gerke, V. A novel ligand of the formyl peptide receptor: annexin I regulates neutrophil extravasation by interacting with the FPR.Molec. Cell 5: 831-840, 2000. |
