Description | Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin-2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation. Angiopoietin 1 binding to Tie 2 causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus. |
Catalog Number | IW-PA1005 |
Quantity | 9 ml |
Host | Rabbit |
Clone | Polyclonal |
Isotype | IgG |
Immunogen | A synthetic peptide corresponding to a sequence mapping near the C-terminal of human angiopoietin-2, different from the related mouse sequence by two amino acids. |
Purity | Immunogen affinity purified |
Conjugate | Unconjugated |
Species Reactivity | Human, mouse, rat. Not tested in other species. |
Positive Control | Placenta |
Cellular Localization | Cytoplasmic |
Form | Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted. |
Storage | Store at 2-8 °C. Do not freeze. |
Applications | IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections. IHC-Tek Epitope Retrieval Solution (IW-1100) is recommended. IHC-Fr: Not tested. ICC: Not tested. |
Limitations | This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user. |
Precautions | Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes. |
References | 1.Kim, I.; Kim, J.-H.; Ryu, Y. S.; Jung, S. H.; Nah, J. J.; Koh, G. Y. : Characterization and expression of a novel alternatively spliced human angiopoietin-2. J. Biol. Chem. 275: 18550-18556, 2000. 2. Maisonpierre, P. C.; Suri, C.; Jones, P. F.; Bartunkova, S.; Wiegand, S. J.; Radziejewski, C.; Compton, D.; McClain, J.; Aldrich, T. H.; Papadopoulos, N.; Daly, T. J.; Davis, S.; Sato, T. N.; Yancopoulos, G. D. : Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis. Science 277: 55-60, 1997. 3.Ward, E. G.; Grosios, K.; Markham, A. F.; Jones, P. F. : Genomic structures of the human angiopoietins show polymorphism in angiopoietin-2. Cytogenet. Cell Genet. 94: 147-154, 2001. |
