Bulk anti-CD45R In Vivo Antibody – Low Endotoxin (RA3-6B2)
Product Benefits:
ichorbio’s anti-CD45R In Vivo Antibody – Low Endotoxin (RA3-6B2) is manufactured in a cGMP compliant facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Target:
CD45R
Clone:
RA3-6B2
Isotype:
Rat IgG2a
Other Names:
Leukocyte common antigen, L-CA, T200, CD45, Receptor-type tyrosine-protein phosphatase C, PTPRC, B220
Uniprot:
P08575
Host:
Rat
Species Reactivity:
Mouse, Human
Specificity:
Anti-CD45R In Vivo Antibody – Low Endotoxin (RA3-6B2) recognizes the B-cell determinant (Mr 220 kD) of the CD45 molecule.
Antigen Distribution:
The CD45R antigen is present on mouse B-cells, B-cell precursors, T cell subset, NK cell subsets .
Background:
CD45R, also known as B220 is a 220 kDa transmembrane protein tyrosine phosphatase expressed on B cells and some subsets of T and NK cells. This antibody is commonly used as a B cell marker as CD45R does play a critical role in TCR and BCR signaling.
Immunogen:
Abelson murine leukemia virus-induced pre-B tumor cells
Concentration:
0.5 mg/ml
Formulation:
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:
Aggregation level ≤ 5%
Aggregation level ≤ 1%
IMPACT Pathogen Test:
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:Mycoplasma sppMycoplasma pulmonisPneumonia virus of miceKilham’s rat virusToolan’s H1 virusRat parvovirusLymphocytic choriomeningitis virusRat cytomegalovirusSendai virusRat coronavirusSialodacryoadenitis virusRat minute virusSeoul virusMouse adenovirusReovirus 3Rat theilovirus
Storage:
Anti-CD45R In Vivo Antibody – Low Endotoxin (RA3-6B2) is stable for at least four (4) weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –80°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications:
Western Blot, Functional Assays, Flow Cytometry, IHC (Frozen), IHC (Paraffin), Immunoprecipitation, Immunofluorescence, ELISA
Application Notes:
Functional Assay: In vivo treatment with anti-CD45R (Clone RA3-6B2) reduces the number of T and B cells and modifies their functional activity.Each investigator should determine their own optimal working dilution for specific applications.
Use:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Isotype Control:
Rat IgG2a In Vivo Isotype Control – Low Endotoxin [1-1] (ICH2244)
Related Antibody:
Antibodies against the same target:
Anti-CD45 In Vivo Antibody – Low Endotoxin [I3/2.3] (ICH1057)
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