Description:Mouse monoclonal antibody (mAb) to H. pylori cytotoxin associated gene A (CagA) protein

Purification:Protein G affinity purified

Product Type:Primary antibody

Target Protein:H. pylori cytotoxin associated gene A protein

Immunogen:A 32KD recombinant protein cloned from the highly immunogenic region of H. pylori cytotoxin associated gene A.

Fusion Myeloma:Sp2/0-Ag14

Specificity:The mAb reacts with H. pylori CagA.

Cross-Reactivity:Cross-reaction with other proteins has not been found.

Host / Isotype:Mouse, IgG1 Kappa

Formulation:Lyophilizedfrom a solution in 0.01M PBS, pH 7.0

Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00mg/mL

Storage: Store at -20^oC

Research Area:Bacteriology

Background:

Helicobacter pylori (H. pylori), a spiral rod shaped gram-negative bacterium, is frequently found in the stomach. In infected populations, 10-20% may develop gastritis and gastric ulcer and 1-2% may develop cancer. The genome of H. pylori isolates from carriers with symptoms contains a 40kb pathogenicity island encoding H. pylori cytotoxin, cytotoxin associated gene A protein (CagA) and other virulence associated factors. CagA is used as a biomarker for virulent H. pylori strains.

Application:

ELISA:Clone 5H10 can be used as capture antibody in sandwich ELISA to detect the recombinant CagA antigen in combination with HRP conjugated anti-CagA clone 5C6, clone 3C10 and clone 3C1. In addition, clone 5H10 coated wells selectively react with cell lysate of CagA containing H. pylori strain when HRP conjugated anti-CagA clone 5C6 is used as detection antibody.

References:

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