EPO

Symansis" purified EPO^hcx has N- and O-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F and a further drop in observed MW on SDS-PAGE after treatment with a glycosidase cocktail. Symansis" purified EPO^hcx consists of 25-55% carbohydrate by weight.

Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1. Bartesaghi S. et al. (2005) Neurotoxicology, 26(5):923-82. Brines M & Cerami A (2005) Nat Rev Neurosci. 6(6): 484-94.3. Engert A (2005) Ann Oncol. 16: 1584-95.