CD33 - Fc Chimera/Siglec-3 - Fc Chimera
Symansis" Siglec-3 - Fchcx Chimera has N-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F. Symansis" Siglec-3 - Fchcx Chimera contains 10-30% carbohydrate by weight. Densitometry of protein isoforms visualized by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein. 1D SDS-PAGE of Symansis" hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation. 1. Varki et al. (2006) Glycobiology 16:1R-27R |