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Symansis/Retinol Binding Protein 4 (RBP4) Rapid Human ELISA Kit/96 Well Kit/ERBPSY

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¥12200.00
货号:ERBPSY
浏览量:127
品牌:Symansis
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Retinol Binding Protein 4 (RBP4) Rapid Human ELISA Kit

Catalog Number: ERBPSYAvailability: In StockUniprot Nu :P02753Species Reactivity :HumanApplications :ELISAAlternative Names:Plasma retinol-binding protein, RET4, PRBP, RBP4, RBP
*Well Kit:
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Retinol Binding Protein 4 (RBP4) Rapid Human ELISA KitDescriptionMSDS

Retinol binding protein 4(RBP4), originally known as a specific transport of retinol in blood, is also a novel inflammatory and insulin resistance marker. Serum RBP4 levels are elevated in insulin-resistant mice and humans with obesity and type 2 diabetes. Animal experiments found that increased secretion of RBP4 might reduces insulin-dependent glucose uptake by muscle tissue by reducing the activity of PI(3)K (phosphoinositide 3-kinase), and increased hepatic glucose output by increasing the expression of the enzyme PEPCK2. Studies suggested that elevated serum RBP4 was associated with components of the metabolic syndrome, including increased body-mass index, waist-to-hip ratio, serum triglyceride levels, and systolic blood pressure and decreased high-density lipoprotein cholesterol levels4. Furthermore, circulating RBP4 concentrations were associated with subclinical cardiovascular disease, which imply that RBP4 could be involved in the development of atherosclerosis.This assay utilises a quantitative sandwich Enzyme-Linked Immunosorbent Assay (ELISA) technique and is designed for the quantitative detection of human RBP4 in plasma or serum in 2.5 hours. A mouse monoclonal antibody specific to RBP4 has been pre-coated onto a micro-titre plate. The user pipettes standards and samples into the wells and any RBP4 present is captured by the immobilised antibody. After washing away any unbound substances a second horseradish peroxidase (HRP)-linked antibody specific to RBP4 is added to the wells. After a final wash step to remove any unbound antibody-enzyme reagent, an HRP substrate solution is added and colour develops in proportion to the amount of RBP4 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured RBP4, the unknown sample concentration can be interpolated from a reference curve included in each assay.

This Human RBP4 ELISA kit is designed for quantification of human RBP4 in serum, plasma and adipocyte extracts or cell culture media. Other biological matrices have not been tested. This kit specifically measures native human RBP4 and has no cross reactivity to mouse or rat RBP4.

Tags:ERBPSY,Plasma retinol-binding protein,RET4,PRBP,RBP4,RBP,P02753
Symansis的抑制剂阵列Easy50™阵列是连续稀释的药物阵列,已知它们专门靶向某些细胞信号分子。这些可直接应用于基于酶或细胞的测定,以达到跨越EC 50  和IC 50 的最终浓度 适合该药物的分子靶标。使用生成的信息,研究人员可以快速确定特定的细胞信号通路是否参与正在研究的生物过程。阵列的形式是一个96孔微孔板,包含12列,每列有一种或多种抑制剂,以及8行,每列包含该抑制剂的渐进稀释液,最后一行除外,该行留空作为阴性对照。对于每种抑制剂范围的稀释液从该抑制剂的IC的大约十分之一50 到一成百倍的IC 50