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Stemrd/Frizzled-5 Fc fusion, human/50 µg/HFZ5FC-050

价格
¥3900.00
货号:HFZ5FC-050
浏览量:127
品牌:Stemrd
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商品描述
SourceCHO cellsM.W.CAS No.
Structural Info
FormulationLyophilized in sterile filtered solution of PBS.
ReconstitutionBefore reconstitution, abrief spin is recommend to drive down any material dislodged from the bottom ofthe tube. The lyophilized protein shouldbe reconstituted in sterile H2O to a desired concentration.
StabilityThe lyophilized protein isstable for at least one year if stored at -80 °C. Reconstituted protein is stable forat least four weeks at 4 °C, but should be stored in aliquots at -80°C for longer term. Avoidrepeated freeze and thaw.
PurityGreater than 95% by SDS-PAGE analysis
Biological ActivityThe activity was determined by using a TCF reporter gene assay in cultured human cells.The IC50 ranges from 100 - 200 ng/ml in the inhibition of 100 ng/mL WNT-3a activity.
Country of OriginUSA

Frizzled-5 is one of the 10 mammalian Frizzled receptors for WNTproteins. The extracellular cycteine rich domain of Frizzleds bind toWNTs with nM affinity. When supplied as recombinant protein, thisextracellular domain of Frizzled-5 acts as a soluble receptor, blocking thebinding of WNTs to Frizzled on the cells.
StemRD’s Frizzled-5 extracellular domain protein is fused to theN-terminus of the Fc domain of human IgG1. This fusion allows stabilizationof the protein in vitro and in vivo and dimerization of the recombinant proteinrendering high-affinity binding to WNTs. A similar protein, Frizzled-8 Fc fusion, has been used byGenentech scientists to show anti-tumor effect of WNT blockade in animal models(DeAlmeida VI et al, Cancer Res. 2007, 67:5371).
Expression is from CHO cells cultured in serum-free and protein-freemedium. Purification of the fusionprotein from conditioned culture medium is performed using protein Achromatography.

Frizzled-5 Fc fusion, human recombinant
Stemrd的Y-27632,(1R,4r)-4-((R)-1-氨乙基)-N-(吡啶-4-基)环己烷-羧酰胺-2HCl,是一种Rho相关激酶(ROCK)抑制剂。Y27632已被证明可以防止凋亡,并提高解离的人类胚胎干(ES)细胞的存活率和克隆效率,而不会影响其多能性。