Source | E. coli | M.W. | 25 kDa | CAS No. | |
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Structural Info | |||||
Formulation | Lyophilized from sterile filtered PBS solution, carrier-free. | ||||
Reconstitution | Before reconstitution, we recommend a brief spin to drivedown any material dislodged from the bottom of the tube. It is recommended to reconstitute the lyophilized product inacid solution such as 1mM HCl. Furtherdilutions should be made in sterile buffer or medium containing carrierproteins, such as albumin or serum. | ||||
Stability | The lyophilized protein is stable for at least 1 yearif stored at -80 °C. Reconstituted protein is stable for at least 2 week at 4 ° C, but shouldbe stored in aliquots at -80 °C for longer term. Avoidrepeated freeze/thaw. | ||||
Purity | Greater than 95% by SDS-PAGE analysis | ||||
Biological Activity | The activity was determined by proliferation of the NIH3T3 cells.The EC50 ranges from 1 – 5 ng/ml. | ||||
Country of Origin | USA |
Platelet-Derived Growth Factor (PDGF) is a mitogen for manycell types, such as fibroblast, smooth muscle cells and endothelial cells. StemRD produces PDGF-bb in Escherichia coli as a disulfide bond-linked homodimer with amolecular mass of 25 kDa. Thisproduct is suitable for tissue culture uses.