Source | M.W. | ~40 kDa | CAS No. | ||
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Structural Info | |||||
Formulation | Lyophilized in sterile filtered solution of PBS with 1% CHAPS | ||||
Reconstitution | Before reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized protein shouldbe reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of thisprotein, further dilutions should be made in buffer or medium containing carrierproteins, such as albumin or serum. | ||||
Stability | The lyophilized protein isstable for at least 1 year if stored at -80 °C. Reconstituted protein is stable for at least 1month at 4 °C, but should be stored in aliquots at -80°C for longerterm. Avoid repeated freeze andthaw. | ||||
Purity | Greater than 90% as determined by SDS-PAGE analysis | ||||
Biological Activity | The activity was determined by using a TCF reporter gene assay in NIH3T3 cells.The EC50 ranges from 50 - 150 ng/ml. | ||||
Country of Origin | USA |
WNT-3a is a member of the WNT family ofsignaling proteins that play a key role in embryonic development and theintegrity of adult tissues. WNT-3a is aprototypic canonical WNT that signals through the b-catenin pathway. The predicted size of mouse WNT-3a is amonomeric protein containing 333 amino acid residues. Due to glycosylation, it migrates at anapparent molecular weight of 40 kDa by SDS-PAGE analysis under non-reducingconditions. StemRD’s product isexpressed from a mouse cell line overexpressing mouse WNT-3a. Purification is performed with a proprietaryprocess that is distinct from the published method.