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商品描述
| Source | M.W. | ~45 kDa | CAS No. | ||
|---|---|---|---|---|---|
| Structural Info | |||||
| Formulation | Lyophilized in sterile filtered solution of PBS with 2% CHAPS | ||||
| Reconstitution | Before reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized protein shouldbe reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of thisprotein, further dilutions should be made in buffer or medium containingcarrier proteins, such as albumin or serum. | ||||
| Stability | The lyophilized protein isstable for at least 1 year if stored at -80 °C. Reconstituted protein is stable for at least1 month at 4 °C, but should be stored in aliquots at -80 °C forlonger term. Avoidrepeated freeze and thaw. | ||||
| Purity | Greater than 85% as determined by SDS-PAGE and HPLC analysis | ||||
| Biological Activity | The activity was determined by using a TCF reporter gene assay in 293 cells co-transfected with Frizzled-4 and LRP-5.WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay. | ||||
| Country of Origin | USA | ||||
WNT-5a belongs to the class of WNT proteins thatactivate the “non-canonical” pathway. Thepredicted size of mouse WNT-5a is a monomeric protein containing 356 amino acidresidues. Due to glycosylation, itmigrates at an apparent molecular weight of ~45 kDa by SDS-PAGE analysis undernon-reducing conditions. StemRD’sproduct is expressed from a mouse cell line, and purified with a proprietaryprocess that is distinct from the published method.
Product Sheet
Ishida-Takagishi M, Enomoto A, etal., The Dishevelled-associating protein Daple controls the non-canonicalWnt/Rac pathway and cell motility. NatureCommunication. 2012May 29;3:859 http://www.ncbi.nlm.nih.gov/pubmed/22643886Nakamuta S, Funahashi Y, Namba T etal., Local application of neurotrophins specifies axons throughinositol 1,4,5-trisphosphate, calcium, and Ca2+/calmodulin-dependentprotein kinases. ScienceSignal. 2011 Nov 15;4(199):ra76.http://www.ncbi.nlm.nih.gov/pubmed/22087032
