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0512-67156496
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商品描述

 

General information

Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.

Kit contents

  1. Champion™ Competent Cells

  2. pUC19 Control Plasmid (5 μl, 10-4 μg/μl)

  3. Champion™ Transformation Protocol Card

Shipping condition

Throughout the shipping process, the temperature is maintained under -70°C. 

Storage and expiration 

Champion™ Competent Cells must be stored between -70°C to -80°C. Subsequent freeze-thaw cycles will reduce transformation efficiency. If high efficiency is required for the experiment, do not use aliquots that have gone through several freeze-thaw cycles. The efficiency of Champion™ Competent Cells lasts for 1 year with proper storage.

 

Genotypes and applications

Product Name

Genotype

Application

Champion™ 109 High

e14(McrA-)recA1 endA1 gyrA96 hsdR17(rk-, mK+) phoA supE44 thi-1 relA1 ∆(lac-proAB) (F’traD36 proAB lacIqZ∆M15

Appropriate for blue-white color and robotic screening. It is a fast growing strain forming visible colonies within 8~10 hours.

Champion™ 21

F’ ompT hsdSβ(rβ-mβ-) dcm gal λ (DE3)

Appropriate host for recombinant protein expression using T7-based expression vectors.

Champion™ DH5α High

recA1 endA1 gyrA96 hsdR17(rk-, mK+) phoA supE44 relA1 thi-1 ∆(lacZYA-argF)U 169 φ80 ∆(lacZ)M15 F’

Suitable for cloning with large plasmid and cDNA library construction, and also for blue-white colony selection.

Items and ordering information

Product Name

Compatible to E. coli strain

Efficiency (cfu/μg)

Quantity

Cat. No.

Champion™ 109 High

E. coli JM109

 >1 x 108

100 μl x 80 vials

CC0202

100 μl x 24 vials

CC0204

Champion™ 21

E. coli BL21 (DE3)

 >1 x 107

100 μl x 80 vials

CC2102

100 μl x 24 vials

CC2104

Champion™ DH5α High

E. coli DH5α

 >3 x 108

100 μl x 80 vials

CC5202

100 μl x 24 vials

CC5204

 

 

Manual

Manual_Champion™ Competent Cell 

SDS

SDS_Champion™ Competent Cell

Flyer

Champion™ Competent Cell

Protocol card

Odoo image and text block

Product Name

Compatible to E. coli strain

Efficiency (cfu/μg)

Quantity

Cat. No.

Champion™ 109 High

E. coli JM109

 >1 x 108

100 μl x 80 vials

CC0202

100 μl x 24 vials

CC0204

Champion 21

E. coli BL21 (DE3)

 >1 x 107

100 μl x 80 vials

CC2102

100 μl x 24 vials

CC2104

Champion DH5α High

E. coli DH5α

 >3 x 108

100 μl x 80 vials

CC5202

100 μl x 24 vials

CC5204

 

Odoo - Sample 1 for three columns

High Fidelity PCR amplification

Amplification of target gene with HiFi™ DNA polymerase to minimize error rate.

  • [TF1000] SMO-HiFi™ DNA Polymerase, (1 U/μl, 100 U) 

  • [TF3000] G-HiFi™ DNA Polymerase, (1 U/μl, 100 U)

Odoo - Sample 2 for three columns

Gel electrophoresis

Staining amplicons with safe fluorescent dyes, following by observation under blue-light illuminator to minimize damage of DNA amplicons and maximize successful cloning efficiency.   

Safe fluorescent dyes

  • [NS1000] FluoroVue™ Nucleic Acid Gel Stain (10,000X), 500 μl

  • [DS1000] FluoroStain™ DNA Fluorescent Staining Dye (Green, 10,000X), 500 μl

  • [DL5000] FluoroDye™ DNA Fluorescent Loading Dye (Green, 6X), 1 ml

Blue-light illuminator

  • [VE0100] B-BOX™ Blue Light LED Epi-illuminator

Odoo - Sample 3 for three columns

Ligation

Blund-end PCR amplicons can directly ligate with PCR cloning vector.  

  • [CV1000] GetClone™ PCR Cloning Vector, 20 Rxn

  • [CV1100] GetClone™ PCR Cloning Vector II, 20 Rxn

Odoo - Sample 3 for three columns

Colony PCR

Analyze colonies with PCR master mix to save preparation time.  

  • [TP1100] ExcelTaq™ 5X PCR Master Mix, 200 Rxn

  • [TP1200] ExcelTaq™ 5X PCR Master Dye Mix, 200 Rxn

2004年,SMOBIO Technology,Inc.迅速出现在生物技术行业。SMOBIO Technology,Inc.首先采用独特的发酵技术,旨在生产高质量功能性食品成分。建立成功的分销网络后,我们将专注于高质量产品继续,我们开发了最先进的生物分子测试工具。我们的工具旨在通过提供以下内容来促进分子生物学和蛋白质科学研究:高质量DNA阶梯和蛋白质标记。这些创新产品已受到业界和学术界人士的高度评价。自2008年以来,SMOBIO Technology,Inc.已展示出非常可观的市场份额。客户满意的证据表明,SMOBIO Technology,Inc.竭尽全力生产高质量产品。我们日益增长的声誉高质量 由超过满意的客户产生的工具已成为周围最好的营销策略之一。