Description 

The ExcelTaq™ 5X/2X PCR Master Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as a master mix for virtually all PCR applications. The mixture contains all components for PCR with the exception of templates and primers. This not only saves valuable time in the laboratory, but also reduces pipetting and reagent handling errors. The PCR Master Mix is supplied as a 5X/2X concentrated ready-to-use mixture of recombinant Taq DNA Polymerase, reaction buffer, MgCl2 (TP1120 contains MgSO4), dNTP, and enzyme stabilizer enabling efficient amplification of template in PCR and allows the user to prepare a PCR reagent conveniently. This product is supplied with 6X DNA Loading Dye (Blue) containing two tracking dyes (Xylene cyanol FF and Bromophenol blue) for post PCR analysis through the use of agarose gel electrophoresis.

Features

• 5’→3’ DNA polymerase activity

• No detectable 3"→5" exonuclease (proofreading) activity

• Generates PCR products with 3"-dA overhangs

• High yield PCR 

• High reproducibility

• Reduced pipetting errors

Applications 

• Routine PCR

• Colony PCR

• High throughput PCR

• Amplification of DNA fragments up to 8 kb

• Generation of PCR products for TA cloning

• DNA labeling

Storage

4°C for 6 months-20°C for 24 months