Description
ExcelTaq™ Klen-Taq DNA Polymerase is a specially blended enzyme mix containing KlenTaq-1 DNA polymerase (a 5’-exo-minus, N-terminal deletion of Taq DNA polymerase) and a small amount of a proofreading DNA polymerase. This unique blending helps to improve the fidelity, yield and processivity of the resultant PCR process. The Klen-Taq is also highly robust, showing high tolerance of varying concentration of Mg2+; it is highly thermostable and has four times the fidelity compared to Taq DNA polymerase. The ExcelTaq Klen-Taq DNA Polymerase is ideal for DNA amplifications 0.5-5 kb in length on genomic DNA, and up to 10 kb on less complex templates.
Features
5"→3" DNA polymerase activity
3"→5" exonuclease activity (proofreading)
Thermo-stable up to 98°C during PCR denaturing step
4× fidelity as compared to Taq DNA polymerase
Robust PCR performance, resistance to variance in PCR condition
Storage
-20°C for 24 months
Elongation capability
ExcelTaq™ Klen-Taq DNA Polymerase can amplify PCR products from λDNA up to 12 kb (M: DM5100).
Sensitivity
ExcelTaq™ Klen-Taq DNA Polymerase can amplify PCR products from as little as 1 pg of template DNA (M: DM2100).
Contents
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ExcelTaq™ Klen-Taq DNA Polymerase mixture
Klen-Taq DNA polymerase 5 U/ µl
A proofreading DNA polymerase Trace
Storage Buffer
40 mM Tris-HCl (pH 7.5), 50 mM KCl, 25 mM (NH4)2SO4, 0.1 mM EDTA, 5.0 mM 2-mercaptoethanol, 50% (v/v) glycerol
10X Klen Buffer
400 mM Tricine-KOH (pH 9.2 at 25°C), 150 mM KOAc, 35 mM Mg(OAc)2, 750 µg/ ml Bovine Serum Albumin
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
Storage
-20°C for 24 months
Manual
Manual_TK1000_ExcelTaq™ Klen-Taq DNA Polymerase
SDS
SDS_TK1000
General Terms and Conditions / Service Policies
Does ExcelTaq™ Klen-Taq DNA Polymerase (TK1000) product have chemical modification or biological modification?
It is without any modification. Moreover, the proof-reading activity of Klen-taq products is thrice that of a normal Taq DNA polymerase.
Is ExcelTaq™ Klen-Taq DNA Polymerase (TK1000) a blended enzyme mix?
Klen-Taq DNA polymerase (TK1000) is blended KlenTaq DNA polymerse and a small amount proofreading enzyme.
What is the end structure of PCR products generated by ExcelTaq™ Klen-Taq DNA Polymerase (TK1000)?
Since ExcelTaq™ Klen-Taq DNA polymerase (TK1000) is blended KlenTaq-1 DNA polymerse and a small amount proofreading enzyme, the end structure of PCR products generated by ExcelTaq™ Klen-Taq DNA Polymerase will be mixture of 3’-dA overhangs and blunt ends.
Recommended PCR Condition
Template | 1– 150 ng |
Forward primer | 0.1– 0.5 µM |
Reverse primer | 0.1– 0.5 µM |
10X Klen Buffer | 5µl |
dNTPs | 0.2mM (each) |
Klen-Taq enzyme | 0.5µl (2.5 U) |
ddH2O | to50 µl |
Total volume | 50µl |
Recommended PCRProgram
Steps | Temp. | Time | Cycles |
Template denature | 94°C | 2 min | 1 |
Denature | 94°C | 30 sec | 25-40 |
Annealing | 50-68°C | 30 sec | |
Extension | 68°C | 30 sec/kb | |
Final extension | 68°C | 1 min | 1 |
[RP1000] ExcelRT™ Reverse Transcriptase
High yield
Thermostable, up to 50°C, during first strand synthesis
High processivity, generating cDNA up to 8 kb
Reduced RNase H ribonuclease activity
[TF1000] SMO-HiFi™ DNA Polymerase
5’→3’ DNA polymerase activity
3’→5’ exonuclease (proofreading) activity
High reaction rate—10 seconds/kb
High fidelity— 70 times higher than Taq polymerase
Generates blunt end amplicon
Thermo-stable for more than 10 hrs at 95°C.
[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)
High sensitivity and signal intensity
Compatible with fast PCR program
With smart blue contrast dye as a visual aid for reaction setup
Low background
High stability
[TP1200] ExcelTaq™ 5X PCR Master Dye Mix
5’→3’ DNA polymerase activity
No detectable 3"→5" exonuclease (proofreading) activity
Generates PCR products with 3"-dA overhangs
High yield PCR
High reproducibility
Reduced pipetting errors
Includes tracking dye for direct loading after PCR
