Description
The SMO-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase with fidelity 70 times higher than Taq DNA polymerase during amplification, as well as very high elongation rate. Being highly thermostable, SMO-HiFi™ DNA Polymerase can remain viable even after being subjected to boiling for 2 minutes. The SMO-HiFi™ DNA Polymerase is also designed to operate in much lower Mg2+ concentration as compared to other DNA polymerase products.
Features
5"→3" DNA polymerase activity
3"→5" exonuclease activity (proofreading)
High reaction rate (up to 1 kb/10 second)
High fidelity, 70 times higher than Taq DNA polymerase
Blunt end amplicon
Thermo-stable-half life is more than 10 hrs at 95°C
Storage
[TF1000] SMO-HiFi™ DNA Polymerase
-20°C for 24 months
Elongation capability
SMO-HiFi™ DNA Polymerase’s high processability enables reliable amplification of λDNA up to 12 kb in length (M: DM5100).
Sensitivity
SMO-HiFi™ DNA Polymerase can amplify PCR products from as little as 1 fg of template DNA (M: DM2000).
[TF1000] SMO-HiFi™ DNA Polymerase
Contents
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Storage Buffer
50 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
Storage
-20°C for 24 months
[TF1000] SMO-HiFi™ DNA Polymerase
Manual
Manual_TF1000_SMO-HiFi™ DNA Polymerase
SDS
SDS_TF1000
What are the differences between SMO-HiFi™ (TF1000) and G-HiFi™ (TF3000)?
SMO-HiFi™ (TF1000) and G-HiFi™ (TF3000) contain different high fidelity DNA polymerases. SMO-HiFi™ (TF1000) and G-HiFi™(TF3000) DNA polymerases are suitable for GC-rich templates that are difficult to amplify. Moreover G-HiFi™ (TF3000) is blended with elongation enhancer to perform quick and accurate PCR.
Recommended PCR Condition [TF1000] SMO-HiFi™ DNA Polymerase
Template | 1– 150 ng |
Forward primer | 0.1– 0.5 µM |
Reverse primer | 0.1– 0.5 µM |
10X HiFi™Buffer | 5µl |
MgSO4 (25 mM) | 2μl |
dNTPs (2 mM each) | 5µl |
SMO-HiFi™DNA Polymerase | 1µl (1 unit) |
DMSO | 5µl |
H2O | to50 µl |
Total volume | 50µl |
Recommended PCR Program
Steps | Temp. | Time | Cycles |
Template denature | 94°C | 2 min | 1 |
Denature | 94°C | 30 sec | 25-40 |
Annealing | 50-68°C | 30 sec | |
Extension | 68°C | 30 sec/kb | |
Final extension | 68°C | 1 min | 1 |
*Optimal PCR condition variesaccording to primers’ thermodynamic properties.
Gel electrophoresis
Staining amplicons with safe fluorescent dyes, following by observation under blue-light illuminator to minimize damage of DNA amplicons and maximize successful cloning efficiency.
Safe fluorescent dyes
[NS1000] FluoroVue™ Nucleic Acid Gel Stain (10,000X), 500 μl
[DS1000] FluoroStain™ DNA Fluorescent Staining Dye (Green, 10,000X), 500 μl
[DL5000] FluoroDye™ DNA Fluorescent Loading Dye (Green, 6X), 1 ml
Blue-light illuminator
[VE0100] B-BOX™ Blue Light LED Epi-illuminator
Ligation
Blund-end PCR amplicons can directly ligate with PCR cloning vector.
[CV1100] GetClone™ PCR Cloning Vector II, 20 Rxn
Transformation
Prepare competent cells with high efficiency and transform with time-saving protocol.
[CK1000] Champion™ E. coli Transformation Kit
Colony PCR
Analyze colonies with PCR master mix to save preparation time.
[TP1100] ExcelTaq™ 5X PCR Master Mix, 200 Rxn
[TP1200] ExcelTaq™ 5X PCR Master Dye Mix, 200 Rxn
