Description
The DM1100 ExcelBand™ 50 bp DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA ladder DM1100 is composed of 17 individual DNA fragments: 1.5k, 1.2k, 1k, 900, 800, 700, 600, 500, 450, 400, 350, 300, 250, 200, 150, 100, and 50 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (500 bp and 200 bp) for easier reference. In addition, the low range Orange G tracking dye mimics the migration of a 50 bp dsDNA during electrophoresis is also added for real time monitoring.
Features
Sharp bands
Quick reference— enhanced bands
Ready-to-use— premixed with loading dye for direct loading
Stable— room temperature storage over 6 months
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
50 ~ 1,500 bp
Concentration
54 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Room temperature for 6 months4°C for 12 months -20°C for 36 months
Specification
Cat. No. | DM1100 |
Series Name | ExcelBand™ |
Product Size | 500 μl |
Size Range | 50 – 1500 bp |
Band Number | 17 |
Tracking Dye | Orange G |
Enhanced Band | 200 and 500 bp |
Manual
Manual_DM1100_ExcelBand™ 50 bp DNA Ladder
SDS
SDS_DM1100
Are the DNA markers/ladders produced by SMOBIO sufficient in quantity?
Yes, all the DNA markers of SMOBIO have been passed in the QC processes including repeated optical density measurements to ensure the quantity of total DNA.
Are the SMOBIO"s DNA markers/ladders compatible to radio-labeling (for example, label DNA with T4 Polynucleotide Kinase)?
We do not recommend to do the labeling reaction directly.
The reasons are as follows:
Our DNA marker is pre-mixed with loading buffer that contains Tris-HCl, EDTA, and glycerol, may affect radio-labeling.
Our DNA marker is a mixture of PCR products and restriction enzyme digested DNA fragments. Digested dsDNA may still have phospho-group on their 5" end.
To enhance the efficiency of the labeling reaction, here are two steps suggested being done prior to the labeling reaction:
Purify DNA marker by EtOH precipitation or DNA purification kits to remove loading buffer.
Remove phospho-group by using phosphatase, ex CIAP (Calf intestinal alkaline phosphatase)
Are SMOBIO"s DNA markers/ladders suitable to use in DNA PAGE?
SMOBIO"s AccuBand™ series DNA markers (DM1200, DM2000, DM2200 and DM2400) are suitable for DNA PAGE.
Why DNA markers/ladders are resolved two bands at the same size when using high percentage agarose or polyacrylamide gel?
DNA fragments with identical in size are indistinguishable on agarose gels, but, on acrylamide gels, even slight differences in DNA sequence can lead to noticeably different migration rates. To provide increased intensity of DNA marker/ladder bands, multiple DNA fragments with identical in size but different in DNA sequences are used. SMOBIO"s AccuBand™ series DNA markers (DM1200, DM2000, DM2200 and DM2400) are more suitable for DNA PAGE.
Are SMOBIO"s DNA markers/ladders suitable to use in denaturing DNA PAGE?
SMOBIO"s AccuBand™ series DNA markers (DM1200, DM2000, DM2200 and DM2400) are suitable for DNA PAGE.
SMOBIO"s AccuBand™ DNA markers are not at denatured form, therefore, you need to denature DNA ladder by yourself.
Here is the protocol to denature DNA ladder:
Mix 5 μL of DNA Ladder with an equal volume of denaturing solution [95% (v/v) formamide, 10 mM EDTA (pH 8.0), 0.1% (w/v) bromophenol blue, 0.1% (w/v) xylene cyanol].
Incubate at 70°C for 5 minutes.
Electrophorese the sample in a denaturing polyacrylamide/urea gel
The conserved basic residues and the charged amino acid residues at the α-helix of the zinc finger motif regulate the nuclear transport activity of triple C2H2 zinc finger proteins
Chih-Ying Lin, Lih-Yuan Lin PLoS One. 2018; 13(1): e0191971. Published online 2018 Jan 30. doi: 10.1371/journal.pone.0191971
PMCID: PMC5790263
ExcelBand™ DNA Ladder series
FluoroBand™ DNA Ladder series
AccuBand™ DNA Marker series
FluoroVue™ Nucleic Acid Gel Stain
Excellent for in-gel staining
Sensitivity up to 0.14 ng DNA or 1 ng total RNA
A safer alternative to EtBr
Suitable to blue or UV light
FluoroStain™ DNA Fluorescent Staining Dye
Excellent for post staining
Sensitivity up to 0.04 ng DNA
A safe alternative to EtBr
Suitable for blue or UV light
FluoroDye™ DNA Fluorescent Loading Dye
Excellent for premix with DNA sample
Sensitivity up to 0.14 ng DNA
Safety dye
Convenience - monitor the electrophoresis in real-time
