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商品描述
CXCL5 ELISA Kit, Human: General Information
Product name
CXCL5 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum,Cell supernatant,Plasma
Sensitivity
1.44 pg/mL
Assay range
4.69-300 pg/mL
Specificity
Recognizes both recombinant and natural Human CXCL5
Interference
Preparations ofthe factors listed below at 50 ng/mL in a mid-range recombinant human CXCL5 control were assayed for interference. No significant interference was observed.
Precision
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 61.94 | 99.46 | 191.27 |
| SD | 2.65 | 5.03 | 7.23 |
| CV(%) | 4.3% | 5.1% | 3.8% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 59.84 | 102.41 | 199.19 |
| SD | 1.48 | 3.62 | 4.33 |
| CV(%) | 2.5% | 3.5% | 2.2% |
Recovery
The recovery of Human CXCL5 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 110 |
| Range |
| 105-119% |
| Sample |
| supernatant (n=3) |
| Average % Recovery |
| 121 |
| Range |
| 112-129% |
| Sample |
| plasma (n=3) |
| Average % Recovery |
| 106 |
| Range |
| 103-112% |
Linearity
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 110% |
| 1:4 | 103% |
| 1:8 | 97% |
| 1:16 | 72% |
Supernatant
| Recovery of detected | |
|---|---|
| 1:2 | 99% |
| 1:4 | 97% |
| 1:8 | 97% |
| 1:16 | 90% |
Plasma
| Recovery of detected | |
|---|---|
| 1:2 | 104% |
| 1:4 | 105% |
| 1:8 | 95% |
| 1:16 | 84% |
Materials provided
1. 96 well microplate coated with Capture Antibody2. Detection Antibody conjugated to HRP3. Standards4. Wash Buffer Concentrate5. Dilution Buffer Concentrate6. Color Reagent A7. Color Reagent B8. Stop Solution
Product overview
This CXCL5 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL5 protein in Serum,Cell supernatant,Plasma . It contains recombinant Human CXCL5, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃Opened/Reconstituted Reagents: Please refer to CoA
CXCL5 ELISA Kit, Human: Images
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CXCL5. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
CXCL5 ELISA Kit, Human: Synonyms
ENA-78 ELISA Kit, Human; SCYB5 ELISA Kit, Human
CXCL5 Background Information
CXCL5 is a small cytokine belonging to the CXC chemokine family. CXC chemokines are particularly significant for leukocyte infiltration in inflammatory diseases. CXCL5 is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. It also can be detected in eosinophils, and can be inhibited with the type II interferon. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis. It stimulates the chemotaxis of neutrophils possesses angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2.
Full Name
chemokine (C-X-C motif) ligand 5
Related Pathways
IL17 signaling pathway
References
- Dawes JM, et al. (2011) CXCL5 Mediates UVB Irradiation-Induced Pain. Sci Transl Med. 3(90): 90ra60.
- O"Donovan N, et al. (1999) Physical mapping of the CXC chemokine locus on human chromosome 4. Cytogenet. Cell Genet. 84(1-2):39-42.
- Persson T, et al. (2003) Expression of the neutrophil-activating CXC chemokine ENA-78/CXCL5 by human eosinophils. Clin Exp Allergy. 33(4):531-7.
- Schwann cells promote lung cancer proliferation by promoting the M2 polarization of macrophagesAuthorZhou, Y;Li, J;Han, B;Zhong, R;Zhong, H;Year2020JournalCellular ImmunologyApplicationELISAFoldedExpand
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