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蚂蚁淘在线 / 品牌中心 / Sino Biological / Sino Biological/CD14 Protein, Mouse, Recombinant (His & hFc Tag)/1/50023-M03H
商品描述
CD14 Protein, Mouse, Recombinant (His & hFc Tag): Product Information
Purity
> 97 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Pro 345) of mouse CD14 (NP_033971.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Accession#
NP_033971.1
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Ser 16
Molecule Mass
The recombinant mouse CD14/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 578 amino acids and has a predicted molecular mass of 63.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD14/Fc monomer is approximately 85-95 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
CD14 Protein, Mouse, Recombinant (His & hFc Tag): Images
CD14 Protein, Mouse, Recombinant (His & hFc Tag): Synonyms
CD14 Protein, Mouse
CD14 Background Information
The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 14 (CD14) is a member of the CD system. It takes its name from its inclusion in the CD molecule surface marker proteins. CD14 exists in two forms: a form anchored into the membrane or a soluble form. CD14 was found expressed in macrophages, neutrophil granulocyte and dendritic cells. The major function is to serve as a co-receptor (along with TLR4 and MD-2) for the bacterial lipopolysaccharide (LPS) and other pathogen-associated molecular patterns.
Full Name
CD14 molecule
Related Pathways
NF-kB (NFkB) Pathway
References
- Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
- Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
- Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
- SD Wright, et al. (1990) CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 249 (4975): 1431-3.
Human CD14 Proteins
- CD14 Protein, Human, Recombinant (His Tag)10073-H08H
- CD14 Protein, Human, Recombinant (hFc Tag)10073-H01H
Mouse CD14 Proteins
- CD14 Protein, Mouse, Recombinant (His Tag)50023-M08H
Rat CD14 Proteins
- CD14 Protein, Rat, Recombinant (His Tag)80308-R08H
- CD14 Protein, Rat, Recombinant (hFc Tag)80308-R02H
Cynomolgus CD14 Proteins
- CD14 Protein, Cynomolgus, Recombinant (His Tag)90174-C08H
- Homeostatic and pathogenic roles of GM3 ganglioside molecular species in TLR4 signaling in obesityAuthorKanoh, H;Nitta, T;Go, S;Inamori, KI;Veillon, L;Nihei, W;Fujii, M;Kabayama, K;Shimoyama, A;Fukase, K;Ohto, U;Shimizu, T;Watanabe, T;Shindo, H;Aoki, S;Sato, K;Nagasaki, M;Yatomi, Y;Komura, N;Ando, H;Ishida, H;Kiso, M;Natori, Y;Yoshimura, Y;Zonca, A;Cattaneo, A;Letizia, M;Ciampa, M;Mauri, L;Prinetti, A;Sonnino, S;Suzuki, A;Inokuchi, JI;Year2020JournalEMBO J.Applicationcell culturePubMed ID: 32378734FoldedExpand
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