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蚂蚁淘在线 / 品牌中心 / Sino Biological / Sino Biological/EphA3 Protein, Human, Recombinant (His Tag)/1/11459-H08H
商品描述
EphA3 Protein, Human, Recombinant (His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its binding ability in a functional ELISA.Immobilized human EPHA3-His at 10 μg/ml (100 μl/well) can bind human EphrinA5-Fc (Cat:10192-H02H), The EC50 of human EphrinA5-Fc (Cat:10192-H02H) is 6.2-14.6 ng/ml.
Protein Construction
A DNA sequence encoding the human EPHA3 (NP_005224.2) (Met1-Gln541) was expressed with a polyhistidine tag at the C-terminus.
Accession#
NP_005224.2
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Glu 21
Molecule Mass
The recombinant human EPHA3 consists of 532 amino acids and predicts a molecular mass of 60.3 KDa. It migrates as an approximately 65-70 KDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
EphA3 Protein, Human, Recombinant (His Tag): Images
Measured by its binding ability in a functional ELISA. Immobilized human EPHA3-His at 10 μg/ml (100 μl/well) can bind human EphrinA5-Fc (Cat: 10192-H02H), The EC50 of human EphrinA5-Fc (Cat: 10192-H02H) is 6.2-14.6 ng/ml.
EphA3 Protein, Human, Recombinant (His Tag): Synonyms
EK4 Protein, Human; ETK Protein, Human; ETK1 Protein, Human; HEK Protein, Human; HEK4 Protein, Human; TYRO4 Protein, Human
EphA3 Background Information
EPHA3 gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA3 gene encodes a protein that binds ephrin-A ligands. EPHA3 is involved in the retinotectal mapping of neurons. It may also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
EPH receptor A3
Related Pathways
Actin Dynamics Signaling Pathway
G Protein-coupled Receptors Signaling
MAPK-Erk Pathway
Autophagy Pathway
References
- Holder N, et al. (1999) Eph receptors and ephrins: effectors of morphogenesis. Development. 126(10):2033-44.
- Wilkinson DG. (2000) Eph receptors and ephrins: regulators of guidance and assembly. Int Rev Cytol. 196:177-244.
- Xu Q, et al. (2001) Roles of Eph receptors and ephrins in segmental patterning. Philos Trans R Soc Lond B Biol Sci. 355(1399):993-1002.
