Product name

TNF-alpha Matched ELISA Antibody Pair Set,Human

Assay type

Solid Phase Sandwich ELISA

Specificity

Quantitative determination of Human TNF-alpha

Assay range

39.06-2500 pg/mL

Materials provided

1. Capture Antibody 1 mg/mL of mouse anti-Human TNF-alpha / TNFA / TNFSF2 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10602-MM01)2. Detection Antibody 0.2 mg/mL of mouse anti-Human TNF-alpha / TNFA / TNFSF2 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.3 μg/mL in Dilution Buffer before use. (Catalog: # 10602-MM08)3. Standard Each vial contains 55 ng of recombinant Human TNF-alpha / TNFA / TNFSF2. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 2500 pg/mL is recommended.

Product overview

This TNF-alpha Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human TNF-alpha . It contains Human TNF-alpha capture antibody, Human TNF-alpha detector antibody (HRP)and a highly purifiedE. coli-expressedrecombinant Human TNF-alpha protein. This Pair Set is at affordable price for researchers.

Shipping

This Matched ELISA Antibody Pair Set is shipped at ambient temperature.

Storage

Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.