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商品描述
PDGFRA ELISA Kit, Human: General Information
Product name
PDGFRA ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
86.73 pg/mL
Assay range
109.38-7000 pg/mL
Specificity
Recognizes both recombinant and natural Human PDGFRA
Precision
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 1,237.59 | 2,512.69 | 5,158.21 |
| SD | 72.70 | 109.99 | 245.24 |
| CV(%) | 5.9% | 4.4% | 4.8% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 1,135.39 | 2,464.60 | 4,891.08 |
| SD | 77.92 | 200.67 | 160.46 |
| CV(%) | 6.9% | 8.1% | 3.3% |
Recovery
The recovery of Human PDGFRA spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 90 |
| Range |
| 83-98% |
Linearity
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 100% |
| 1:4 | 112% |
| 1:8 | 107% |
| 1:16 | 96% |
Materials provided
1. 96 well microplate coated with Capture Antibody2. Detection Antibody conjugated to HRP3. Standards4. Wash Buffer Concentrate5. Dilution Buffer Concentrate6. Color Reagent A7. Color Reagent B8. Stop Solution
Product overview
This PDGFRA ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGFRA protein in Serum . It contains recombinant Human PDGFRA, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃Opened/Reconstituted Reagents: Please refer to CoA
PDGFRA ELISA Kit, Human: Images
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human PDGFRA. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
PDGFRA ELISA Kit, Human: Synonyms
CD140A ELISA Kit, Human; PDGFR-2 ELISA Kit, Human; PDGFR2 ELISA Kit, Human; RHEPDGFRA ELISA Kit, Human
PDGFRA Background Information
PDGFRA, also known as CD140a, together with the structurally homolog protein PDGFRB (CD140b), are cell surface receptors for members of the platelet-derived growth factor family. They are members of the class III subfamily of receptor tyrosine kinase (RTKs) with the similar structure characteristics of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGFRA is expressed in oligodendrocyte progenitor cells and mesothelial cell, and binds all three ligand isoforms PDGF-AA, PDGF-BB and PDGF-AB with high affinity, whereas PDGFRB dose not bind PDGF-AA. PDGFRA plays an essential role in regulating proliferation, chemotaxis and migration of mesangial cells. Recent studies have indicated that PDGFRA acts as a critical mediator of signaling in testis organogenesis and Leydig cell differentiation, and in addition, particularly important for kidney development. Additionally, PDGFRA is involved in tumor angiogenesis and maintenance of the tumor microenvironment and has been implicated in development and metastasis of Hepatocellular carcinoma (HCC). PDGFRA may represent a potential therapeutic target in thymic tumours. PDGFRA gene amplification rather than gene mutation may be the underlying genetic mechanism driving PDGFRA overexpression in a portion of gliomas.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
platelet-derived growth factor receptor, alpha polypeptide
Research Areas
- Cancer Drug Targets
- Receptor Tyrosine Kinases (RTKs)
References
- Oseini AM, et al. (2009) PDGFRalpha: a new therapeutic target in the treatment of hepatocellular carcinoma? Expert Opin Ther Targets. 13(4): 443-54.
- Meister M, et al. (2009) Expression and mutational status of PDGFR in thymic tumours. Anticancer Res. 29(10): 4057-61.
- Martinho O, et al. (2009) Expression, mutation and copy number analysis of platelet-derived growth factor receptor A (PDGFRA) and its ligand PDGFA in gliomas. Br J Cancer. 101(6): 973-82.
- Comparative evaluation of mesenchymal stromal cells from umbilical cord and amniotic membrane in xeno-free conditionsAuthorMu, Y;Wu, X;Hao, Z;Year2018JournalBMC Cell Biol.ApplicationELISAPubMed ID: 30545286FoldedExpand
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