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1. Measured by its binding ability in a functional ELISA.2. Immobilized human CSF1R (Cat:10161-H08H) at 10 μg/mL (100 μl/well) can bindbiotinylated human CSF-1 (Cat:11792-H08H).The EC₅₀ of biotinylated human CSF-1 (Cat:11792-H08H) is 8 ng/mL.3. Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells.The ED₅₀ for this effect is typically 2-8 ng/mL.

Protein Construction

A DNA sequence encoding the N-terminal fragment (Met 1-Asn 190) of human / cynomolgus CSF1 (P09603-1) was fused with a polyhistidine tag at the C-terminus. Human and Cynomolgus CSF1 sequences are identical.

Accession#

P09603-1

Expressed Host

HEK293 Cells

Species

Human, Cynomolgus

Predicted N Terminal

Glu 22

Molecule Mass

The recombinant human / cynomolgus CSF1 consists of 169 amino acids and predictes a molecular mass of 19.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCSF1 is approximately 27-32 kDa band due to glycosylation.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hard copy of CoA.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

M-CSF Protein, Human, Cynomolgus, Recombinant (His Tag): Images

Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells.The _ED50 for this effect is typically 2-8 ng/mL.

M-CSF Background Information

Macrophage colony-stimulating factor1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membraneprotein which is disulfide-linked as a homodimeror heterodimer. Granulocyte / macrophagecolony-stimulating factors are cytokines that act in hematopoiesis by controlling the production,differentiation, and function of 2 related white cell populations of the blood,the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known tofacilitate monocyte survival, monocyte-to-macrophage conversion, andmacrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate intomacrophages or other related cell types. It binds to the Colony stimulatingfactor 1 receptor. M-CSF/CSF-1 may also be involved in development of theplacenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-boundprecursors. M-CSF/CSF-1 induces cells of the monocyte/macrophagelineage. It also plays a role in immunological defenses, bone metabolism,lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarctedmyocardium may have an active role in healing not only through its effects oncells of monocyte/macrophage lineage, but also byregulating endothelial cell chemokine expression.

Full Name

colony stimulating factor 1 (macrophage)

References

Pandit J. et al., 1992, Science. 258: 1358-62.
Tokai M. et al., 2000, J Bacteriol. 182(10): 2865-8.
Fan X. et al., 2001, Am J Physiol Endocrinol Metab. 280(1): E103-11.
Frangogiannis NG. et al., 2003, Am J Physiol Heart Circ Physiol. 285 (2): H483-92.
Cupp JS. et al., 2007,Am J Surg Pathol. 31(6): 970-6.

Human M-CSF Proteins

M-CSF Protein, Human, Recombinant (His Tag)11792-H08Y
M-CSF Protein, Human, Recombinant, Biotinylated11792-HNAH-B
M-CSF Protein, Human, Recombinant11792-HNAH
M-CSF Protein, Human, Recombinant (hFc Tag)11792-H02H
M-CSF Protein, Human, Recombinant (His Tag)11792-H08H1

Mouse M-CSF Proteins

M-CSF Protein, Mouse, Recombinant (ECD)51112-MNAH
M-CSF Protein, Mouse, Recombinant (His Tag)51112-M08H

Human, Cynomolgus M-CSF Proteins

M-CSF Protein, Human, Cynomolgus, Recombinant (His Tag), Biotinylated11792-H08H-B

Cynomolgus, Rhesus M-CSF Proteins

M-CSF Protein, Cynomolgus, Rhesus, Recombinant (hFc Tag)90029-C02H
M-CSF Protein, Cynomolgus, Rhesus, Recombinant (His Tag)90029-C08H

A potent in vivo anti-tumor efficacy of novel recombinant type I interferon
Author
Zhang, K;Yin, XF;Yang, YQ;Li, HL;Xu, YN;Chen, LY;Liu, XJ;Yuan, SJ;Fang, XL;Xiao, J;Wu, S;Xu, H;Chu, L;Katlinski, KV;Katlinskaya, YV;Guo, RB;Wei, GW;Wang, DC;Liu, X;Fuchs, SY;
Year
2016
Journal
Clin. Cancer Res.
Application
binding
FoldedExpand
A potent in vivo anti-tumor efficacy of novel recombinant type I interferon
Author
Zhang, K;Yin, XF;Yang, YQ;Li, HL;Xu, YN;Chen, LY;Liu, XJ;Yuan, SJ;Fang, XL;Xiao, J;Wu, S;Xu, H;Chu, L;Katlinski, KV;Katlinskaya, YV;Guo, RB;Wei, GW;Wang, DC;Liu, X;Fuchs, SY;
Year
2016
Journal
Clin. Cancer Res.
Application
binding
FoldedExpand

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