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蚂蚁淘在线 / 品牌中心 / Sino Biological / Sino Biological/B7-1 Protein, Human, Recombinant (ECD, His Tag)/1/10698-H08H
商品描述
B7-1 Protein, Human, Recombinant (ECD, His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its binding ability in a functional ELISA. Immobilized B7-1 Protein, Human, Recombinant (His Tag) (Cat: 10698-H08H) at 2 μg/ml (100 μl/well) can bind CTLA-4 Protein, Human, Recombinant (His & hFc Tag) (Cat: 11159-H03H). The EC50 of of CTLA-4 (His & hFc Tag) (Cat: 11159-H03H) is 35-80 ng/ml.
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Asn 242) of human B7-1 (NP_005182.1) precursor was fused with a polyhistidine tag at the C-terminus.
Accession#
NP_005182.1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Val 35
Molecule Mass
The recombinant human B7-1 consists of 219 amino acids and has a predicted molecular mass of 25.4 kDa. By reduced SDS-PAGE, the apparent molecular mass of rh B7-1 is approximately 45-48 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
B7-1 Protein, Human, Recombinant (ECD, His Tag): Images
Measured by its binding ability in a functional ELISA. Immobilized B7-1 Protein, Human, Recombinant (His Tag) (Cat: 10698-H08H) at 2 μg/ml (100 μl/well) can bind CTLA-4 Protein, Human, Recombinant (His & hFc Tag) (Cat: 11159-H03H). The EC50 of of CTLA-4 (His & hFc Tag) (Cat: 11159-H03H) is 35-80 ng/ml.
B7-1 Protein, Human, Recombinant (ECD, His Tag): Synonyms
B7 Protein, Human; B7-1 Protein, Human; B7.1 Protein, Human; BB1 Protein, Human; CD28LG Protein, Human; CD28LG1 Protein, Human; LAB7 Protein, Human
B7-1 Background Information
The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
- Cancer Immunotherapy
- Co-inhibitory Immune Checkpoint Targets
- Immune Checkpoint
- Immune Checkpoint Detection: Antibodies
- Immune Checkpoint Detection: ELISA Antibodies
- Immune Checkpoint Detection: FCM Antibodies
- Immune Checkpoint Detection: IHC Antibodies
- Immune Checkpoint Detection: WB Antibodies
- Immune Checkpoint Proteins
- Immune Checkpoint Targets
- Immunotherapy
- Targeted Therapy
Full Name
CD80 molecule
Research Areas
- Cancer Drug Targets
References
- Greenfield EA, et al. (1998) CD28/B7 costimulation: a review. Crit Rev Immunol. 18(5): 389-418.
- Zang X, et al. (2007) The B7 family and cancer therapy: costimulation and coinhibition. Clin Cancer Res. 13(18 Pt 1): 5271-9.
- Mir MA, et al. (2008) Signaling through CD80: an approach for treating lymphomas. Expert Opin Ther Targets. 12(8): 969-79.
Human B7-1 Proteins
- B7-1 Protein, Human, Recombinant (ECD, His & hFc Tag)10698-H03H
- B7-1 Protein, Human, Recombinant (ECD, hFc Tag)10698-H02H
- B7-1 Protein, Human, Recombinant10698-HCCH
- B7-1 Protein, Human, Recombinant (His Tag), Biotinylated10698-H08H-B
Mouse B7-1 Proteins
- B7-1 Protein, Mouse, Recombinant (His Tag), Biotinylated50446-M08H-B
- B7-1 Protein, Mouse, Recombinant (His Tag)50446-M08H
- B7-1 Protein, Mouse, Recombinant (hFc Tag)50446-M02H
Rat B7-1 Proteins
- B7-1 Protein, Rat, Recombinant (ECD, hFc Tag)80027-R02H
Cynomolgus B7-1 Proteins
- B7-1 Protein, Cynomolgus, Recombinant (His Tag)90268-C08H
- B7-1 Protein, Cynomolgus, Recombinant (hFc Tag)90268-C02H
- B7-1 Protein, Cynomolgus, Recombinant (His Tag), HPLC-verifiedHPLC-90268-C08H
- The utility of urinary CD80 as a diagnostic marker in patients with renal diseasesAuthorMinamikawa, S;Nozu, K;Maeta, S;Yamamura, T;Year2018JournalScientific reportsApplicationELISA (standard)PubMed ID: 30470792FoldedExpand
- PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 PathwaysAuthorZhao, Y;Lee, CK;Lin, CH;Gassen, RB;Xu, X;Huang, Z;Xiao, C;Bonorino, C;Lu, LF;Bui, JD;Hui, E;Year2019JournalImmunityPubMed ID: 31757674FoldedExpand
- A functional antibody cross-reactive to both human and murine cytotoxic T-lymphocyte-associated protein 4 via binding to an N-glycosylation epitopeAuthorLi, D;Li, J;Chu, H;Wang, Z;Year2020JournalMAbsApplicationELISAPubMed ID: 32054416FoldedExpand
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