Cell Information:

In addition to functioning as physical barriers against microbes, the urethral epithelial linings participate in the first line of host defense by production of a variety of proinflammatory mediators when exposed to microbes and microbial agents. This might occur via adhesion-mediated signalling by receptor interaction or via attachment-facilitated presentation of conserved microbial patterns to TLRs on epithelial cells.

Primary Human Urethra Epithelial Cell (HUEpC) is a good in vitro model for pathogenesis studies of urethral epithelial cells in various diseases.

The Transfection Reagent:

Transfection of primary cells, such as HUEpC, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Urethra Epithelial Cell (HUEpC) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HUEpC.  The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HUEpC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series

Features:

• Unique Formulation-Maximize transfection performance in HUEpC
• Extremely gentle to cells
• Single or multiple plasmids transfection
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines.
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells.
• Compatible with serum
• Developed and manufactured by EZ Biosystems

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