Cell Line Information:

Designations: WI-38
Depositors: L Hayflick
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: lung
Disease: normal
Cell Type: fibroblast
Applications: Transfection host; viruscide testing
Cytogenetic Analysis:normal diploid
Isoenzymes: G6PD, B
Age: 3 months gestation fetus
Gender: female
Ethnicity: Caucasian
The WI-38 human diploid cell line was derived by Leonard Hayflick from normal embryonic (3 months gestation) lung tissue. WI-38 cells have a finite lifetime of 50 plus or minus 10 population doublings with a doubling time of 24 hours. This line was the first human diploid cell line to be used in human vaccine preparation.

The Transfection Reagent:

WI-38 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on WI-38 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for WI-38 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of WI-38 Cell Avalanche™ Transfection Reagent for transfection on WI-38 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in WI-38 Cells.
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on WI-38 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for WI-38 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this WI-38 Cell Avalanche Transfection Reagent.   FIG. 2. WI-38 cells were transfected with GFP vector (pEGFP-N3) by using WI-38 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

WI-38 Cell Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies. Caki-1 Cell MDCK Cell Vero Cell 293 Cell  MRC-5 Cell IMR-90 Cell COS-7 Cell BHK-21 Cell 3197-3 Cell NIH/3T3 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200