The MiQuant® Residual DNA dPCR HEK-293 is designed for the quantitative detection of residual DNA from HEK-293 cells in chemical and biological matrices.

The MiQuant® Residual DNA dPCR HEK-293 is designed for use with the QIAGEN® QIAcuity® digital PCR (dPCR) system as a novel rapid, robust and sensitive method for the detection of residual host cell DNA.

Type of PCR
Digital PCR (dPCR)

Recommended Use
To test product quality, the amount of residual DNA in a drug"s final dosage form must follow guidelines established by the World Health Organization (WHO), the U.S. Food and Drug Administration (FDA), the European Pharmacopoeia, and other regulatory agencies.

Flyer

Kit Components
    dPCR HEK-293 Mix in aliquots of 24 reactions each
    Rehydration Buffer
    Positive Control DNA
    Internal Control DNA
    dPCR Grade Water

Package Size
Cat. No. 58-0121:
24 reactions

Cat. No. 58-0122:
4 x 24 reactions

Result Evaluation
Absolute Quantification

Required Consumables
    PCR reaction tubes
    PCR Clean™ (15-2025, 15-2001)
    PCR reaction tubes or plates
    MiQuant® dPCR Validation Standard HEK-293 (Cat. No. 58-0221)

Required Lab Device
    QIAcuity® digital PCR cycle
    QIAcuity® 24Well 26K Nanoplates
    Microcentrifuge for PCR reaction tubes
    Pipetting equipment

Shelf Life and Storage
The expiry date of the unopened package is marked on the package label. The kit components must be stored at2 to8°C until use. The rehydrated components must be store at ≤ -18°C for a maximum of one month.

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