POLY-PAK™ PURIFICATION

The use of Poly-Pak™ packings in cartridges or barrels overcomes several disadvantages usually associated with reverse phase (RP) cartridges. The packing is stable in the pH range 1-13, thus the ammonium hydroxide solution, diluted with water, is loaded directly onto the packing. Also, after elution of failure sequences, the trityl group is removed and washed from the support-bound oligonucleotide. The fully deprotected product can then be eluted and isolated by lyophilization. Poly-Pak™ Cartridges may also be used for desalting normal or labelled oligonucleotides. The original Poly-Pak cartridge and barrel are designed for 0.2 µmole syntheses or less. Poly-Pak II cartridges and barrels are designed for use with 1 µmole syntheses. A booklet, User Guide To Poly-Pak™ Cartridge Purification, describes in detail the process and several applications. This booklet is delivered with cartridges, barrels, and packing and is available on request.

GLEN-PAK™ PURIFICATION

Glen-Pak? DNA and RNA cartridges have advantages over Poly-Pak cartridges in that a single loading of the diluted crude deprotection solution is all that is necessary. Also, the range of purification has been extended to 100using DMT-on oligos. Glen-Pak cartridges have similar performance to Fluoro-Pak cartridges but without the need for the fluorous DMT group at the 5" terminus and special phosphoramidites, so the cost is lower. In addition, Glen-Pak cartridges allow purification of virtually the complete range of dyes and modifiers. The Glen-Pak DNA Cartridge 3g is a large cartridge capable of purifying 10-20 µmole oligonucleotide syntheses using the standard DMT-on procedure and Glen-Pak DNA 30mg 96-Well Plates are for parallel purification of up to 50 nmole scale syntheses. Scale suggestions for the Glen-Pak DNA product line are shown below:

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