品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com

scarabgenomics/pSX2 Expression Vector/P-3330

价格
面议
货号:P-3330
浏览量:127
品牌:scarabgenomics
服务
全国联保
正品保证
正规发票
签订合同
商品描述

Background

ScarabXpress®-2 system, our second-generation protein expression system, consists of the pSX2 expression plasmid and ANY Clean Genome® E. coli host strain. In side by side comparisons, the ScarabXpress®-2 system protein expression system gave up to 36x higher yields than standard expression vectors with wild-type E. coli strains

Figures

Figure 1. pSX2 Expression Plasmid Map. Figure 2. 3000-Fold Induction Range. IPTG Titration of pSX2-GFP, intensity was measured, replicates were averaged and graphed in average fluorescent units. Figure 3. ScarabXpress2 Produces ≤ 36x More Protein Than BL21(DE3). Figure 4. More Consistent Fermentations With ScarabXpress®-2

Specifications

Kit Components pSX2 Expression Vector, 10 μg Quality Control The vector is confirmed to be IS-free and for functionality. Storage Conditions Store at ≤ -70°C. Do not store in a frost-free freezer.

Related Products

10X Modified Korz Medium Kit White Glove IS Detection Kit MDS™42 Chemically Competent Cell Kit MDS™42 ΔrecA Chemically Competent Cell Kit MDS™42 ΔrecA Blue Chemically Competent Cell Kit MDS™42 Combination Package Chemically Competent Cell Kit ScarabXpress® T7 lac Chemically Competent Cell Kit MDS™42 Electrocompetent Cell Kit MDS™42 ΔrecA Electrocompetent Cell Kit MDS™42 ΔrecA Blue Electrocompetent Cell Kit MDS™42 ΔrecA trfA Electrocompetent Cell Kit MDS™42 ΔrecA trfA Blue Electrocompetent Cell Kit MDS™42 Combination Package Electrocompetent Cell Kit

Support

Product Manuals pSX2 Expression Vector

Patents & Disclaimers

Scarab is providing you with this Material subject to the non-transferable right to use the subject amount of the Material for your research at your academic institution. The Recipient agrees not to sell or otherwise transfer this Material, or anything derived or produced from the Material to a third party. NO RIGHTS ARE PROVIDED TO USE THE MATERIAL OR ANYTHING DERIVED OR PRODUCED FROM THE MATERIAL FOR COMMERCIAL PURPOSES. If the Recipient makes any changes to the chromosome of the Material that results in an invention in breach of this limited license, then Scarab will have a worldwide, exclusive, royalty-free license to such invention whether patentable or not. If the Recipient is not willing to accept the terms of this limited license, Scarab is willing to accept return of this product with a full refund, minus shipping and handling costs. For information on obtaining a license to this Material for purposes other than research, please contact Scarab’s Licensing Department. Scarab Genomics’ technology is covered by U.S. Pat. No. 6,989,265 and related foreign applications. Clean Genome® is a registered trademark of Scarab Genomics, LLC.

scarabgenomics的D-0710-100-10倍改良Korz培养基,100毫升 ***** 1个D-0710-1LK-10X改良Korz培养基,1000毫升 ***** 1个可重现的结果 –使用已知所有成分的特定培养基,而不是包含酵母提取物或酪蛋白水解物的不确定培养基,可以使结果紧密复制。降低监管负担 -由于Korz不包含任何动物来源的成分,因此不需要原产地证明。降低风险 –消除不确定的成分可大大降低与复杂生物相关的过程相关杂质的风险。为了简化基本培养基的使用,圣甲虫基因组公司提供了一个包含10倍改良Korz培养基和单独的50倍硫酸镁溶液的两组分试剂盒。将浓缩的基本培养基和相关的硫酸镁溶液稀释以创建1X培养基。然后必须添加碳源以支持细胞生长。我们建议使用相当于0.2%的葡萄糖。稀释的1X培养基(含碳源和适当的抗生素)用于摇瓶中的表达优化。在分批补料发酵中,相同的培养基也可用作“分批”阶段的培养基。通常,将碳源的水平调节至比摇瓶中使用的水平更高的水平,例如,当使用葡萄糖时,葡萄糖水平将增加至0.5%。圣甲虫的CleanGenome®菌株是专门为生产生物治疗性蛋白质和DNA而设计的。用于生物治疗生产的“最清洁”培养基是化学定义的最小培养基。因此,改良的Korz基本培养基已使用Scarab CleanGenome®菌株进行了广泛测试,以验证其支持细胞生长和重组蛋白生产的能力。Korz基本培养基最初被设计用于大肠杆菌的高密度分批补料发酵(Korz 等。1995)。培养基由磷酸盐缓冲液,镁,柠檬酸铁,微量元素组成。用户需要提供碳源。用于优化摇瓶表达的相同基础培养基也可用于分批补料发酵,从而在两个过程之间提供连续性。在分批补料发酵中,相同的培养基仅补充了较高的碳源含量。