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蚂蚁淘在线 / 品牌中心 / PromoCell / PromoCell/Cancer Cell Line Medium XF

PromoCell/Cancer Cell Line Medium XF

价格
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货号：C-28077
浏览量：127
品牌：PromoCell
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商品描述

The PromoCell Cancer Cell Line Medium XF is a cell culture medium developed for the standardized in vitro cultivation of established human cancer cell lines. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials. Thus, it has no ill-defined components such as fetal calf serum, extracts or hydrolysates and exhibits very low lot-to-lot variability.

Components:The PromoCell Cancer Cell Line Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium

Note: Culture vessels need to be coated. For coating we recommend using Human Fibronectin Solution (C-43060) or Vitronectin (C-69201).

Key features:

  • Suitable for long-term routine culture of adherently growing established human cancer cell lines
  • Compatible with most commonly used human cancer cell lines
  • Xeno-free and serum-free formulation

List of cell types tested for serial passage with the PromoCell Cancer Cell Line Medium XF:

TissueTested Cell LinesCell Lines OriginRemarks
BrainBV2immortalized murine primary microglial cellsCoat with Fibronectin:1 µg/cm2
BreastMCF-7pleural effusion of metastatic human breast adenocarcinomaCoat with Fibronectin:1 µg/cm2
ColonHT-29human colon adenocarcinomaCoat with Vitronectin:0.5 µg/cm2
Connective tissueHT1080human fibrosarcomaCoat with Fibronectin:1 µg/cm2
LiverHepG2hepatocellular carcinoma of the human liverCoat with Vitronectin:0.5 µg/cm2
LungA-549human lung carcinomaCoat with Vitronectin:0.5 µg/cm2
ProstateLNCaPlymph node metastasis of human prostate adenocarcinoma3D culture in C-28070 is recommended
Peripheral bloodMV-4-11Human acute myelogenous leukemia (suspension)No coating required
Bone marrowKG-1Human acute myelogenous leukemia (suspension)No coating required
KidneyACHNHuman Renal Cell CarcinomaCoat with Fibronectin:1 µg/cm2
BrainA172Human GlioblastomaCoat with Fibronectin:1 µg/cm2
Skeletal muscleC2C12Mouse MyoblastsCoat with Fibronectin:1 µg/cm2
SkinB16-F10Mouse MelanomaCoat with Fibronectin:1 µg/cm2
Abelson murine leukemia virus-induced tumorRAW264.7Mouse MacrophageCoat with Fibronectin:1 µg/cm2
Brain, neuroectodermalNeuro-2aMouse NeuroblastomaCoat with Fibronectin:1 µg/cm2
Figure 1. This figure shows example growth curves of four frequently used cancer cell lines cultivated in Cancer Cell Line Medium XF (C-28077), a serum- and xeno-free culture system. All of them exhibited a constant proliferation rate. The cell lines were plated at 5,000 cells/cm2 in T25 flasks coated with appropriate attachment substrates. For HT1080, 1 µg/cm2 of human fibronectin was used, while A549, HT29, and HepG2 were seeded on 0.5 µg/cm2 of recombinant human vitronectin. The cells were subcultured for between four and seven days depending on the cell line’s proliferation rate. The cumulative population doublings of 10 subsequent passages are shown for each cell line.
Figure 1. This figure shows example growth curves of four frequently used cancer cell lines cultivated in Cancer Cell Line Medium XF (C-28077), a serum- and xeno-free culture system. All of them exhibited a constant proliferation rate. The cell lines were plated at 5,000 cells/cm2 in T25 flasks coated with appropriate attachment substrates. For HT1080, 1 µg/cm2 of human fibronectin was used, while A549, HT29, and HepG2 were seeded on 0.5 µg/cm2 of recombinant human vitronectin. The cells were subcultured for between four and seven days depending on the cell line’s proliferation rate. The cumulative population doublings of 10 subsequent passages are shown for each cell line.
Figure 2. Phase-contrast images of various cell lines cultured in serum- and xeno-free Cancer Cell Line Medium XF (C-28077). The cell lines robustly maintained their typical morphological phenotypes while exhibiting faster proliferation rates that with conventional serum-containing culture systems. The magnifications of the images shown are A: 100x, B: 40x, C: 100x, D: 40x, E: 100x, and F: 100x.
Figure 2. Phase-contrast images of various cell lines cultured in serum- and xeno-free Cancer Cell Line Medium XF (C-28077). The cell lines robustly maintained their typical morphological phenotypes while exhibiting faster proliferation rates that with conventional serum-containing culture systems. The magnifications of the images shown are A: 100x, B: 40x, C: 100x, D: 40x, E: 100x, and F: 100x.
PromoCell支原体安全细胞培养物污染是一个普遍存在的问题，会影响细胞的许多生化和免疫学特性，并且会导致结果不令人满意和不可靠。因此，定期测试细胞培养物中是否存在支原体或细菌污染是必不可少的。PromoCell为此提供了高度灵敏和用户友好的测试套件，以及有效消除和预防支原体和真细菌污染的产品。