品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述

PromoCell Mononuclear Cells (MNC) represent the lymphocyte and monocyte fraction isolated from umbilical cord blood or peripheral blood. They are prepared by optimized low-density gradient centrifugation, which effectively reduces the granulocyte content. Red blood cells are gently but thoroughly depleted by proprietary techniques instead of osmotic lysis. This results in ultra-pure mononuclear cells with superior viability and unchanged biological function. In addition, the cells do not clump after thawing, allowing for easy handling and reproducible results.

The MNC can be used directly for experiments or as a starting material for the isolation of several cell types, e.g., hematopoietic and endothelial progenitors, monocytes, and lymphocyte subpopulations.

NEW: Our hMNC are now also available from HLA-typed donors.

Available formats:

  • Cryopreserved: Cryogenic vial containing 25.000.000 viable cells.
  • Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Recommended plating density1,000,000 cells per ml
Tested MarkersCD31 negative, CD34 negative, CD105 positive, CD146 positive
Figure 1. Flow cytometric analysis of Mononuclear Cells from Cord Blood (hMNC-CB). Granulocytes content 1%.
Figure 1. Flow cytometric analysis of Mononuclear Cells from Cord Blood (hMNC-CB). Granulocytes content 1%.
Figure 2. Flow cytometric determination of CD34cells within the PromoCell Mononuclear Cells from Cord Blood (hMNC-CB)
Figure 2. Flow cytometric determination of CD34cells within the PromoCell Mononuclear Cells from Cord Blood (hMNC-CB)
PromoCell支原体安全细胞培养物污染是一个普遍存在的问题,会影响细胞的许多生化和免疫学特性,并且会导致结果不令人满意和不可靠。因此,定期测试细胞培养物中是否存在支原体或细菌污染是必不可少的。PromoCell为此提供了高度灵敏和用户友好的测试套件,以及有效消除和预防支原体和真细菌污染的产品。