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蚂蚁淘在线 / 品牌中心 / PromoCell / PromoCell/Human M2 Macrophages (M-CSF) monocyte-derived

PromoCell/Human M2 Macrophages (M-CSF) monocyte-derived

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面议
货号：C-12915
浏览量：127
品牌：PromoCell
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商品描述

PromoCell’s crypreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.

Cryopreserved human macrophages are produced from monocytes in PromoCell’s well-proven M2 Macrophage Generation Media DXF and are available as non-activated, fully qualified M2- (M-CSF) polarized cells. After 9-10 days of differentiation, the M2 macrophages are cryopreserved using PromoCells’s proprietary, defined and animal-free freezing medium, Cryo-SFM. Each cryo vial contains more than 1.5 million or 5 million viable cells after thawing, respectively.

NEW: Our M2 Macrophages are now also available from HLA-typed donors.

Recommended plating density200,000 cells per cm2
Tested MarkersCD163 positive, CD68 positive
CryoMacrophages an Overview
Figure 1. Culture of PromoCell"s cryopreserved human macrophages. The macrophages can be directly used for experiments after thawing and plating. Optional: activation of polarized M1-/M2 macrophages 24h before assay.
CryoMacrophages an Overview
Figure 1. Culture of PromoCell"s cryopreserved human macrophages. The macrophages can be directly used for experiments after thawing and plating. Optional: activation of polarized M1-/M2 macrophages 24h before assay.
CryoMacrophages-FACS
Figure 2. Flow cytometric analysis of PromoCell cryopreserved Human M1 and M2 Macrophages. M1 macrophages exhibit a CD68+ (99,74%) and CD80+ (83,33%) marker expression profile, typical for M1 macrophages (upper row). M2 macrophages exhibit a CD68+ (99,72%) and CD163+ (94,91%) marker expression profile, typical for M2 macrophages (lower row).
CryoMacrophages-FACS
Figure 2. Flow cytometric analysis of PromoCell cryopreserved Human M1 and M2 Macrophages. M1 macrophages exhibit a CD68+ (99,74%) and CD80+ (83,33%) marker expression profile, typical for M1 macrophages (upper row). M2 macrophages exhibit a CD68+ (99,72%) and CD163+ (94,91%) marker expression profile, typical for M2 macrophages (lower row).
PromoCell支原体安全细胞培养物污染是一个普遍存在的问题，会影响细胞的许多生化和免疫学特性，并且会导致结果不令人满意和不可靠。因此，定期测试细胞培养物中是否存在支原体或细菌污染是必不可少的。PromoCell为此提供了高度灵敏和用户友好的测试套件，以及有效消除和预防支原体和真细菌污染的产品。